Highly sensitive method for determination of N-nitrosamines using high-performance liquid chromatography with online UV irradiation and luminol chemiluminescence detection.
Highly sensitive method for determination of N-nitrosamines using high-performance liquid chromatography with online UV irradiation and luminol chemiluminescence detection.
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DOI：
10.1016/j.chroma.2008.11.025
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发表时间：
2009-01
期刊：
影响因子：
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通讯作者：
Hitoshi Kodamatani;S. Yamazaki;Keiitsu Saito;Abena Amponsaa-Karikari;N. Kishikawa;N. Kuroda;T. Tomiyasu;Y. Komatsu
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文献类型：
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作者：
Hitoshi Kodamatani;S. Yamazaki;Keiitsu Saito;Abena Amponsaa-Karikari;N. Kishikawa;N. Kuroda;T. Tomiyasu;Y. Komatsu
建立了一种无需预富集步骤即可测定水样中万亿分之一浓度N-亚硝胺的新方法。该方法是基于高效液相色谱分离后的在线紫外光照射和随后的鲁米诺化学发光检测，而不添加氧化剂。证实了碱性水溶液中的N-亚硝胺在紫外光照射下转化为过氧亚硝酸根。在信噪比为3的条件下，N-二甲基亚硝胺、N-亚硝基吗啉、N-亚硝甲基乙胺和N-亚硝基吡咯烷的检出限分别为1.5 ng/L、2.9 ng/L、3.0 ng/L和2.7 ng/L。N-亚硝胺的线性范围为5-1000 ng/L。该方法用于自来水、河水和工业废水中N-亚硝胺的测定。当自来水中N-亚硝基二甲胺、N-亚硝基吗啉、N-亚硝甲基乙胺和N-亚硝基吡咯烷的浓度为10 ng/L(平均±标准偏差，n=4)时，其回收率分别为(94.8±2.7)%、(102.0±6.9)%、(99.3±3.9)%和(102.8±2.5)%。结果表明，该方法可满意地用于水样中N-亚硝胺的测定。
A new method for the measurement of N-nitrosamines in part-per-trillion concentrations from water samples without preconcentration steps has been developed. This method is based on online UV irradiation after high-performance liquid chromatographic separation and subsequent luminol chemiluminescence detection without addition of an oxidant. It was confirmed that N-nitrosamines in basic aqueous solution were transformed to peroxynitrite by UV irradiation. The detection limits for this method were 1.5ng/L, 2.9ng/L, 3.0ng/L, and 2.7ng/L for N-nitrosodimethylamine, N-nitrosomorpholine, N-nitrosomethylethylamine, and N-nitrosopyrrolidine, respectively, at a signal-to-noise ratio of 3. The calibration graphs were linear in the range of 5–1000ng/L for these N-nitrosamines. This method was used for the determination of N-nitrosamines in tap water, river water, and industrial plant effluent samples. The recoveries of N-nitrosodimethylamine, N-nitrosomorpholine, N-nitrosomethylethylamine, and N-nitrosopyrrolidine present in tap water sample at a concentration of 10ng/L (mean±standard deviation, n=4) were (94.8±2.7)%, (102.0±6.9)%, (99.3±3.9)%, and (102.8±2.5)%, respectively. These results indicate that our proposed method can be applied satisfactorily to the determination of N-nitrosamines in water samples.