Maturation of MicroRNA Is Hormonally Regulated by a Nuclear Receptor (Retracted article. See vol. 54, pg. 536, 2014)
Maturation of MicroRNA Is Hormonally Regulated by a Nuclear Receptor (Retracted article. See vol. 54, pg. 536, 2014)
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DOI：
10.1016/j.molcel.2009.08.017
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发表时间：
2009-10-23
期刊：
影响因子：
16
通讯作者：
Kato, Shigeaki
中科院分区：
文献类型：
--
作者：
Yamagata, Kaoru;Fujiyama, Sally;Kato, Shigeaki
类固醇激素及其同源核受体通过调节转录和转录后过程发挥广泛的生物学作用。然而，类固醇激素控制转录后过程的潜在分子机制在很大程度上是未知的。我们现在报道雌激素受体α(ERα)抑制特定的microRNA(MiRNA)的成熟，从而通过3‘UTR稳定ERα靶基因的mRNA。雌激素结合的ERα下调了动物和培养细胞中一组miRNAs的表达。激活的ERα通过雌激素依赖的DROSHA复合体与DROSHA复合体结合，减弱了初级miRNAs向前miRNAs的加工，导致ERα靶基因的转录通过其3‘UTR稳定下来。因此，类固醇激素通过调节miRNA的成熟来实现转录后控制。
Steroid hormones and their cognate nuclear receptors exert a wide spectrum of biological actions through regulation of transcriptional and posttranscriptional processes. However, the underlying molecular mechanism by which steroid hormones control posttranscriptional processes is largely unknown. We now report that estrogen receptor alpha (ER alpha) inhibits the maturation of a particular microRNA (miRNA) and thereby stabilizes the mRNA of an ER alpha target gene through the 3'UTR. Estrogen-bound ER alpha downregulated expression of a set of miRNAs in both animals and cultured cells. Activated ER alpha attenuated the processing of primary miRNAs into pre-miRNAs through estrogen-dependent association with the Drosha complex, resulting in stabilization of the transcript of an ER alpha target gene through its 3'UTR. Thus, a steroid hormone achieves posttranscriptional control by regulating the maturation of miRNA.