Molecular recorders to track cellular events
Molecular recorders to track cellular events
复制标题
分子记录仪追踪细胞事件
DOI：
10.1126/science.abo3471
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发表时间：
2022
期刊：
影响因子：
56.9
通讯作者：
Yachie Nozomu
中科院分区：
文献类型：
--
作者：
Masuyama Nanami;Konno Naoki;Yachie Nozomu
从受精卵开始，细胞增殖，将其基因组信息传递给后代，并动态改变其功能以形成不同的组织结构。在整个发育过程中，细胞内和环境线索触发基因表达模式，这些模式控制细胞状态转换并产生进一步的细胞和环境线索，导致细胞在空间上不同的区域中自组织成功能簇。如何调查这些过程？细胞的高分辨率分子快照可以通过各种组学技术获得，但这些技术需要破坏样品，这妨碍了随着时间的推移进行分析。使用荧光探针的活细胞成像可以分析时程动力学，但仅限于少量分子或细胞。这些挑战可以通过DNA事件记录来克服，其中分子和细胞信息逐渐存储在多细胞生物体内的细胞中的合成“DNA带”中，并且通过高通量DNA测序来访问存储的信息。然而，有一些技术限制需要克服。
Starting from a fertilized egg, cells proliferate, pass their genomic information to their progeny, and dynamically change their functions to form diverse tissue structures. Throughout development, intracellular and environmental cues trigger patterns of gene expression that govern cell state transitions and produce further cellular and environmental cues, leading cells to self-organize into functional clusters in spatially distinct areas. How can these processes be investigated? High-resolution molecular snapshots of cells can be obtained by various omics technologies, but these require sample destruction, which precludes analyses over time. Live-cell imaging with fluorescent probes can analyze time-course dynamics but is limited to a small number of molecules or cells. These challenges could be overcome by DNA event recording, where molecular and cellular information is progressively stored in synthetic “DNA tapes” in cells within a multicellular organism, and the stored information is accessed by high-throughput DNA sequencing. However, there are technical limitations that need to be overcome.