Target-Selective Fluorescence Imaging and Photocytotoxicity against H2O2 High-Expressing Cancer Cells Using a Photoactivatable Theranostic Agent
Target-Selective Fluorescence Imaging and Photocytotoxicity against H2O2 High-Expressing Cancer Cells Using a Photoactivatable Theranostic Agent
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使用光激活治疗诊断剂对 H2O2 高表达癌细胞进行靶点选择性荧光成像和光细胞毒性
DOI：
10.1002/asia.201701004
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Toshima Kazunobu
中科院分区：
文献类型：
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作者：
Takagi Ryoma;Takeda Ayano;Takahashi Daisuke;Toshima Kazunobu
设计合成了一种具有H_2O_2活性的光敏剂1，它由1)芳基硼酸部分 ，2) 前荧光团部分，和3) 光活化部分(光敏剂)组成，在中性水条件下选择性有效地与H_2O_2反应，同时释放间苯二酚进行荧光检测。此外，1是细胞渗透性的，只有在光照射下才表现出对荧光可见细胞的有效光细胞毒作用。结果还表明，1对过氧化氢有选择性的荧光反应，即使在活的培养细胞中也是如此。
A purpose‐designed and synthesized H2O2‐reactive and photoactivatable theranostic agent1consisting of 1) an arylboronic acid moiety, 2) pro‐fluorophore moiety, and 3) photoactivatable moiety (photosensitizer), selectively and effectively reacted with H2O2while simultaneously releasing resorufin for fluorescence detection under neutral aqueous conditions. In addition,1was cell‐permeable, and exhibited effective photocytotoxicity against fluorescently visualized cells only upon photoirradiation. The results also showed that1produced a selective fluorescence response to H2O2, even in living cultured cells.