Distribution of methane production and methanogenic archaeal community structure across soil particle size fractions along a rice chronosequence
Distribution of methane production and methanogenic archaeal community structure across soil particle size fractions along a rice chronosequence
复制标题
甲烷产量和产甲烷古菌群落结构在土壤颗粒尺寸部分沿水稻时间序列的分布
DOI：
10.2489/jswc.74.3.235
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发表时间：
2019-05-01
影响因子：
3.9
通讯作者：
Wang, J.
中科院分区：
文献类型：
--
作者：
Liu, Y.;Dong, Y.;Wang, J.
产甲烷菌是古细菌中的一个系统发育类群，是土壤中重要的微生物群落，在土壤碳循环和甲烷产生中起着重要作用。本研究以水稻（Oryza sativa L.）为研究对象，分析了不同土壤颗粒组分的甲烷产生潜力和古菌群落结构。长期种植水稻的土壤。测定了我国东部沿海地区50 ~ 700年水稻土形成的5个年龄组的年代序列。未受干扰的散装土壤进行了分级，通过低能量超声波分散和离心成四类颗粒大小的部分（PSF;粗砂，细砂，粉砂，粘土）。分别采用定量聚合酶链反应和变性梯度凝胶电泳分析了4个组分中古菌群落的丰度和多样性。其中的大小部分，高丰度的古菌和产甲烷菌被发现在粗砂和粘土大小的部分。在长期的水稻栽培过程中，CH 4生产潜力（MPP）和产甲烷基因丰度增加了100年的土壤耕作，此后或多或少的变化，在较长时期的土壤耕作。此外，基因丰度和社区多样性的显着变化中观察到粗砂和粘土大小的部分，但不是在细砂和粉砂大小的部分。61个选定的古细菌16 S rRNA基因的测序分析表明，甲烷胞菌是一个丰富的组产甲烷菌在所有大小级分的土壤中的年代序列，而Methanocellaceae和Methanosaetaceae是不太普遍的粘土级分在整个土壤。本研究表明，甲烷菌及其活性可以不均匀地分布在土壤中的PSFs，其分布和活性的变化响应于影响PSFs形成的水稻种植年限。
Methanogens, a phylogenetic group of archaea, are a key microbial community that mediate carbon (C) cycling and methane (CH4) production in submerged soils. In this study, we analyzed the potential of CH4 production and the archaeal community structure of different soil particle fractions in a chronosequence of rice (Oryza sativa L.) soil subjected to prolonged rice cultivation. The chronosequence of five age groups of a paddy soil formation was determined in a coastal area from East China, subjected to 50 to 700 years of rice cultivation. Undisturbed bulk soils were fractionated by low-energy ultra-sonication dispersion and centrifugation into four classes of particle size fractions (PSFs; coarse sand, fine sand, silt, and clay). The abundance and diversity of archaeal communities from each of the four fractions were analyzed by quantitative polymerase chain reaction and denaturing gradient gel electrophoresis, respectively. Among the size fractions, a high abundance of archaea and methanogens was found in both coarse sand-and clay-sized fractions. During prolonged rice cultivation, CH4 production potential (MPP) and methanogenic gene abundance increased in the soil cultivated for 100 years, and thereafter changed more or less in the soils cultivated for longer periods. Moreover, marked changes in gene abundance and community diversity were observed in coarse sand- and clay-sized fractions, but not in fine sand- and silt-sized fractions. Sequencing analysis of 61 selected archaeal 16S rRNA genes revealed that Methanocellales was an abundant group of methanogens in all sized fractions of soils across the chronosequence, whereas Methanosarcinaceae and Methanosaetaceae were less widespread in the clay fractions across the soils. This study indicated that methanogens and their activities can be heterogeneously distributed among soil PSFs, and their distribution and activities change in response to years of paddy cultivation that affect PSFs formation.