Differentially transcribed genes in skeletal muscle of Duroc and Taoyuan pigs.
Differentially transcribed genes in skeletal muscle of Duroc and Taoyuan pigs.
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DOI：
10.2527/2005.8392075x
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发表时间：
2005-09
影响因子：
3.3
通讯作者：
C. S. Lin;C. Hsu
中科院分区：
文献类型：
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作者：
C. S. Lin;C. Hsu
本研究的目的是比较两个猪种，杜洛克和桃园之间的LM基因转录谱，显示显着不同的出生后肌肉生长。本研究从新生猪中分离出LM，杜洛克猪的肌长和肌质量均显著大于桃园猪（P < 0.01），但肌纤维面积和肌纤维类型百分比差异不显著，利用由9，182个探针组成的人类高密度互补DNA（cDNA）基因芯片比较了两个品种LM的基因转录谱。结果表明，杜洛克LM中有73个基因的转录水平上调，44个基因的转录水平下调，分别是桃园猪的1.75倍（P < 0.05）。杜洛克猪中强烈上调的基因包括那些编码肌原纤维蛋白复合物的基因（例如，肌球蛋白轻链和重链，以及肌钙蛋白），核糖体蛋白，转录调节蛋白（例如，骨骼肌LIM蛋白1 [SLIM 1]和高迁移率族蛋白），和能量代谢酶（例如，电子转移黄素蛋白脱氢酶、NADH脱氢酶、苹果酸脱氢酶和ATP脱氢酶）。编码能量代谢酶的高度转录的基因表明杜洛克LM中的糖酵解代谢更多，从而有利于碳水化合物而不是脂质用作该组织中的能量底物。编码骨骼肌主导蛋白或控制肌发生和/或肌肉生长的转录调节因子的过转录基因表明了杜洛克猪出生后肌肉生长速率较高的一般机制。其中SLIM 1基因在杜洛克LM猪出生时和出生后7 d的转录水平显著高于桃园猪（P < 0.01）。从初生到7日龄，杜洛克LM猪SLIM 1基因的转录水平显著升高（P < 0.05），而桃园猪SLIM 1基因的转录水平基本不变。这些结果表明，SLIM 1可能是有用的标记与猪出生后肌肉生长的发展。
The objective of this study was to compare gene transcription profiles of LM between two pig breeds, Duroc and Taoyuan, which display dramatically different postnatal muscle growth. We isolated LM from neonatal pigs, and the Duroc muscle length and mass were greater (P < 0.01) than for Taoyuan pigs; however, insignificant differences in the muscle fiber area and the percentage of fiber types were found. A human high-density complementary DNA (cDNA) microarray consisting of 9,182 probes was used to compare gene transcription profiles of LM between the two breeds. The results showed that the transcription level of 73 genes and 44 genes in Duroc LM were upregulated and down-regulated by at least 1.75-fold (P < 0.05) compared with Taoyuan, respectively. The strongly upregulated genes in Duroc pigs included those encoding the complex of myofibrillar proteins (e.g., myosin light and heavy chains, and troponin), ribosomal proteins, transcription regulatory proteins (e.g., skeletal muscle LIM protein 1 [SLIM1] and high-mobility group proteins), and energy metabolic enzymes (e.g., electron-transferring flavo-protein dehydrogenase, NADH dehydrogenase, malate dehydrogenase, and ATP synthases). The highly transcribed genes that encode energy metabolic enzymes indicate a more glycolytic metabolism in Duroc LM, thereby favoring carbohydrates rather than lipids for use as energy substrates in this tissue. The over-transcribed genes that encode skeletal muscle-predominant proteins or transcription regulators that control myogenesis and/or muscle growth suggest a general mechanism for the observed higher rate of postnatal muscle growth in Duroc pigs. The transcription of one such gene, SLIM1, was more highly transcribed (P < 0.01) in Duroc LM at birth and at postnatal d 7 than in Taoyuan. The transcription of SLIM1 increased (P < 0.05) in Duroc LM from neonate through 7 d of age, whereas its transcription remained essentially constant in Taoyuan during this period. These results suggest that SLIM1 may be useful for the development of markers associated with the postnatal muscle growth of pigs.