Lipidic cubic phases as matrices for membrane protein crystallization
Lipidic cubic phases as matrices for membrane protein crystallization
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DOI：
10.1016/j.ymeth.2004.03.030
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发表时间：
2004-11-01
期刊：
影响因子：
4.8
通讯作者：
Nollert, P
中科院分区：
文献类型：
--
作者：
Nollert, P
本文综述了膜蛋白的结晶过程，通过介晶立方相方法。提供了细菌视紫红质特异性的实际操作方案，用于（i）通过在辛基葡糖苷中单体化和凝胶过滤色谱法或通过用十二烷基-三甲基溴化铵预处理后的选择性提取从紫膜制备细菌视紫红质，（ii）通过在小瓶中或在偶联的注射器内混合将细菌视紫红质掺入无水立方相中，（iii）通过添加固体盐或用溶液覆盖使细菌视紫红质在所述基质中结晶。为了给生物化学家和晶体学家提供生长膜蛋白细菌视紫红质晶体所需的所有信息，列出了进一步有用的程序和材料的参考文献。(C)2004年爱思唯尔公司All rights reserved.
This review provides detailed procedures for the crystallization of membrane proteins via the lipidic cubic phase method. Bacteriorhodopsin-specific, hands-on protocols are given for (i) the preparation of bacteriorhodopsin from purple membrane by monomerization in octylglucoside and gel filtration chromatography or by selective extraction after pre-treatment with dodecyl-trimethylammonium bromide, (ii) the incorporation of bacteriorhodopsin into lipidic cubic phases by mixing in vials or within coupled syringes and, (iii) the crystallization of bacteriorhodopsin in the lipidic matrix by adding a solid salt or an overlaying with a solution. References for further useful procedures and materials are listed in order to provide biochemists and crystallographers with all information that is necessary to grow crystals of the membrane protein bacteriorhodopsin. (C) 2004 Elsevier Inc. All rights reserved.