Divergence of spatial gene expression profiles following species-specific gene duplications in human and mouse
Divergence of spatial gene expression profiles following species-specific gene duplications in human and mouse
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DOI：
10.1101/gr.2705204
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发表时间：
2004-10-01
期刊：
影响因子：
7
通讯作者：
Wolfe, KH
中科院分区：
文献类型：
--
作者：
Huminiecki, L;Wolfe, KH
为了研究重复基因在功能上分化的过程，我们研究了人类和小鼠的同源基因组的基因表达谱如何受到最近物种特异性相似物的影响。利用基因表达图谱的微阵列数据，比较了人类和小鼠16个同源组织的基因表达谱，这些数据来自1575组同源物，其中250组具有物种特异性的同源物。我们发现，与人类和小鼠之间保持一对一关系的同源基因相比，最近经历重复的同源基因不太可能具有强烈的相关表达谱。随着基因家族大小的增加，副同源基因在表达模式上变得更加专门化，表达的广度减少，特异性增加。尽管有这种趋势，但对某些特定基因家族的详细检查表明，在这些基因家族中发生了物种特异性的复制，有几个例子显示了复制基因的明显新功能化，但只有一个亚功能化的例子。通常，一个复制基因的两个拷贝的表达似乎相对于祖先的状态发生了变化。我们的研究结果表明，基因表达谱具有惊人的不稳定性，即使在小基因家族的进化过程中，特定组织中的表达也可能反复获得或丢失。我们得出结论，基因复制是不同基因表达模式出现背后的主要驱动力。
To examine the process by which duplicated genes diverge in function, we studied how the gene expression profiles of orthologous gene sets in human and mouse are affected by the presence of additional recent species-specific paralogs. Gene expression profiles were compared across 16 homologous tissues in human and mouse using microarray data from the Gene Expression Atlas for 1575 sets of orthologs including 250 with species-specific paralogs. We find that orthologs that have undergone recent duplication are less likely to have strongly correlated expression profiles than those that remain in a one-to-one relationship between human and mouse. There is a general trend for paralogous genes to become more specialized in their expression patterns, with decreased breadth and increased specificity of expression as gene family size increases. Despite this trend, detailed examination of some particular gene families where species-specific duplications have Occurred indicated several examples of apparent neofunctionalization of duplicated genes, but only one case of subfunctionalization. Often, the expression of both copies of a duplicated gene appears to have changed relative to the ancestral state. Our results suggest that gene expression profiles are Surprisingly labile and that expression in a particular tissue may be gained or lost repeatedly during the evolution of even small gene families. We conclude that gene duplication is a major driving force behind the emergence of divergent gene expression patterns.