Radiation-induced apoptosis of human peripheral T cells: analyses with cDNA expression arrays and mitochondrial membrane potential assay.
Radiation-induced apoptosis of human peripheral T cells: analyses with cDNA expression arrays and mitochondrial membrane potential assay.
复制标题
辐射诱导的人外周 T 细胞凋亡：利用 cDNA 表达阵列和线粒体膜电位测定进行分析。
DOI：
10.3892/ijmm.7.6.603
复制
发表时间：
2001
影响因子：
5.4
通讯作者：
S. Yoshida
中科院分区：
文献类型：
--
作者：
Y. Ogawa;A. Nishioka;T. Kobayashi;S. Kariya;S. Hamasato;T. Saibara;H. Seguchi;S. Yoshida
我们通过使用靶向 Atlas cDNA 表达阵列和线粒体膜电位测定，检查了取自正常志愿者的辐射后外周血 T 细胞的连续变化。 5 Gy 照射后 1 小时和 3 小时，使用细胞凋亡阵列通过靶向 Atlas cDNA 表达阵列检查基因表达的变化。 Atlas Human Apoptosis Array 包含 205 个已知控制细胞凋亡的关键基因，包括细胞外和细胞质效应子。对于Fas，在5 Gy 照射后1 小时和3 小时，照射的T 细胞和未照射的T 细胞之间的斑点强度没有显着变化。 Caspase 家族，包括 Caspase 9 和 3，在这两组之间也没有显示出变化。凋亡调节剂 bclw 显示受辐射的 T 细胞显着减少。这些结果表明，辐射通过改变线粒体膜电位诱导 T 细胞直接凋亡。使用配备 CCD 摄像头的荧光显微镜和线粒体膜电位指示剂 MitoCapture，我们证明了 5 Gy 辐射在辐射后 10 小时会诱导人外周血 T 细胞膜电位损失，即细胞凋亡的早期阶段。
We examined sequential changes in post-irradiated peripheral blood T cells taken from normal volunteers, by using targeted Atlas cDNA Expression Arrays and mitochondrial membrane potential assay. At 1 and 3 hours after 5 Gy irradiation, changes of gene expression were examined by targeted Atlas cDNA Expression Arrays using Apoptosis Array. The Atlas Human Apoptosis Array includes 205 key genes that are known to control apoptosis, including extracellular and cytoplasmic effectors. Concerning Fas, no significant changes of spot intensities were identified between irradiated T cells and non-irradiated ones at both 1 h and 3 h after 5 Gy irradiation. Caspase families, including caspases 9 and 3 also showed no changes between these two groups. An apoptosis regulator bclw showed a remarkable decrease in irradiated T cells. These results suggested that irradiation induced direct apoptosis of T cells by changing the membrane potential of mitochondria. Using a CCD camera-equipped fluorescence microscope and MitoCapture, a mitochondrial membrane potential indicator, we demonstrated 5 Gy radiation induced loss of membrane potential, i.e., an early stage of apoptosis, in human peripheral blood T cells at 10 hours after irradiation.