Pursuing extreme sensitivity for determination of endogenous brassinosteroids through direct fishing from plant matrices and eliminating most interferences with boronate affinity magnetic nanoparticles
Pursuing extreme sensitivity for determination of endogenous brassinosteroids through direct fishing from plant matrices and eliminating most interferences with boronate affinity magnetic nanoparticles
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通过直接从植物基质中捕获并消除硼酸亲和磁性纳米颗粒的大部分干扰，追求内源油菜素类固醇测定的极高灵敏度
DOI：
10.1007/s00216-017-0777-9
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发表时间：
2018-02-01
影响因子：
4.3
通讯作者：
Chu, Jinfang
中科院分区：
文献类型：
--
作者：
Xin, Peiyong;Li, Bingbing;Chu, Jinfang
类维生素B1是调节植物生长发育的重要植物激素。高性能的分析方法，定量内源性BR是重要的研究BR行动的分子机制。在此，我们开发了一种基于硼酸盐亲和磁性纳米粒子（BAMNPs）的高性能样品前处理方法。硼酸盐亲和力的高特异性使得能够从植物基质中直接捕捞BR。强结合能使其能够去除植物基质中的大多数污染物，而目标BR的损失很小。除了这些优点之外，新的两步氧化-水解洗脱系统将BR回收率提高到70.5%-98.2%，这远高于其他硼酸盐亲和应用。最终洗脱液的高清洁度将基质效应降低至85.2%-92.4%。结果表明，该方法能够同时实现内源BR的良好回收和基质干扰的彻底去除，大大提高了BR分析的灵敏度，并将常规分析中使用的植物材料减少到<10 mg。此外，由于BAMNPs操作方便，易于与植物粉末分离，样品处理时间可缩短至<3 h。基于BAMNP固相萃取技术的这些优点，对油菜和水稻组织中BR分布的器官特异性分析显示出良好的灵敏度，通过直接从植物基质中钓取油菜素甾醇（BR），用气相色谱-质谱联用技术消除大部分基质干扰，建立了一种高灵敏度、省时的BR定量方法。制备了硼酸盐亲和磁性纳米颗粒（BAMNPs）。
Brassinosteroids (BRs) are important plant hormones regulating plant growth and development. High-performance analytical methods for quantifying endogenous BRs are important for studying the molecular mechanisms of BR action. Herein we developed a high-performance sample pretreatment method based on boronate affinity magnetic nanoparticles (BAMNPs). The high specificity of boronate affinity enables direct fishing of BRs from plant matrices. The strong binding energy makes it possible to remove most contaminants in plant matrices with a small loss of target BRs. Besides these advantages, the novel two-step oxidation-hydrolysis elution system raised BR recoveries to 70.5%–98.2%, which was much higher than other boronate affinity applications. The high cleanliness of the final eluents lowered the matrix effects to 85.2%–92.4%. As a result, this method enables simultaneously good recoveries of endogenous BRs and thorough removal of matrix interferences, which greatly improves the sensitivity of BR analysis and reduces the use of plant materials for routine analysis to <10 mg. In addition, the sample handling time can be shortened to <3 h due to the operating convenience of BAMNPs and their easy separation from plant powders. Based on these advantages of BAMNP solid phase extraction, the organ-specific BR distribution analysis inArabidopsisand rice tissues demonstrates excellent sensitivity, good reproducibility and high throughput of the method.Graphical abstractA high-sensitivity and time-saving UPLC-MS/MS-based quantification method for brassinosteroids (BRs) was developed through directly fishing BRs from plant matrices and eliminating most matrix interferences with as-prepared boronate affinity magnetic nanoparticles (BAMNPs).