Clinical Evaluation of Fullerene-C60 Dissolved in Squalane for Anti-Wrinkle Cosmetics
Clinical Evaluation of Fullerene-C60 Dissolved in Squalane for Anti-Wrinkle Cosmetics
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DOI：
10.1166/jnn.2010.3053
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发表时间：
2010-10-01
影响因子：
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通讯作者：
Miwa, Nobuhiko
中科院分区：
文献类型：
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作者：
Kato, Shinya;Taira, Hikaru;Miwa, Nobuhiko
将高度纯化且不含有机溶剂的富勒烯-C-60以278 ppm的接近饱和浓度溶解在由橄榄油制备的角鲨烷中，其被命名为LipoFullerene（LF-SQ），并检查其用作具有抗氧化能力的化妆品成分。本研究的目的是评估LF-SQ在受试者中的抗皱形成功效。共有23名日本女性（I组：年龄38.9 ± 3.8，n = 11，II组：年龄39.4 ± 4.3，n = 12）参加了一项为期8周的LF-SQ混合霜试验，该试验是一项随机配对双盲研究。LF-SQ霜剂每天两次施用在右或左半边脸上，角鲨烷混合霜剂（不含富勒烯-C60）作为安慰剂施用在另半边脸上。作为皱纹等级的临床评价，在基线（0周）和第4周和第8周时对两个鱼尾纹区域进行目视观察和拍照，并进行硅胶复制。使用光学轮廓术技术分析皮肤复制品。计算皱纹和皮肤表面粗糙度特征并进行统计分析。随后，通过仪器分析在脸颊上测量经表皮水分损失（TEWL）、角质层的水分水平和粘弹性（柔软度：R 0和弹性：R7）。LF-SO霜能增强皮肤的保湿性和抗皱性。每天两次涂于面部的LF-SQ霜在第4周时无效，但在第8周时显著优于安慰剂（p < 0.05），且无严重副作用。与0周时相比，8周时LF-SQ乳膏的粗糙面积比显示出显著改善（p < 0.05），但LF-SQ乳膏与安慰剂之间没有检测到显著差异。我们建议LF-SQ可以作为一种活性成分用于女性护理化妆品。
Highly purified and organic solvent-free fullerene-C-60 was dissolved, at nearly saturated concentration of 278 ppm, in squalane prepared from olive oil, which is designated as LipoFullerene (LF-SQ) and was examined for usage as a cosmetic ingredient with antioxidant ability. The aim of this study was to assess the anti-wrinkle formation efficacy of LF-SQ in subjects. A total of 23 Japanese women (group I: age 38.9 +/- 3.8, n = 11, group II; age 39.4 +/- 4.3, n = 12) were enrolled in an 8-week trial of LF-SQ blended cream in a randomized, matched pair double-blind study. The LF-SQ cream was applied twice daily on the right or left half of the face, and squalane blended cream (without fullerene-C60) was applied as the placebo on another half of the face. As clinical evaluations of wrinkle grades, visual observation and photographs, and silicone replicas of both crow's feet areas were taken at baseline (0 week) and at 4th and 8th weeks. Skin replicas were analyzed using an optical profilometry technique. The wrinkle and skin-surface roughness features were calculated and statistically analyzed. Subsequently, trans-epidermal water loss (TEWL), moisture levels of the stratum corneum, and visco-elasticity (suppleness: R0 and elasticity: R7) were measured on cheeks by instrumental analysis. LF-SO cream enhanced the skin moisture and the anti-wrinkle formation. LF-SQ cream that was applied on a face twice daily was not effective at 4th week, but significantly more effective than the placebo at 8th week (p < 0.05) without severe side effects. The roughness-area ratio showed significant improvement (p < 0.05) at 8th week with LF-SQ cream as compared to 0 week with LF-SQ cream, but no significant difference was detected between LF-SQ cream and the placebo. We suggest that LF-SQ could be used as an active ingredient for wrinkle-care cosmetics.