Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil.
Cytotoxic Effect and TLC Bioautography-Guided Approach to Detect Health Properties of Amazonian Hedyosmum sprucei Essential Oil.
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DOI：
10.1155/2016/1638342
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Scalvenzi L
中科院分区：
文献类型：
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作者：
Guerrini A;Sacchetti G;Grandini A;Spagnoletti A;Asanza M;Scalvenzi L
生物自显影技术是一种快速、简便的方法，用于检测和鉴定从未研究过的云杉花索尔姆斯（金粟兰科）精油（EO）中的生物活性组分/分子。通过DPPH生物自显影测定和分光光度法评价（IC 50 = 230 ± 10 µg/mL）进行的抗氧化活性似乎主要是由于α-钙地醇和α-木洛醇。（HP）TLC生物自显影，重点是抗菌能力，指出α-杜松醇、α-木洛醇、τ-木洛醇、氧化caraleylene和甲基丁香酚是对金黄色葡萄球菌（被认为是测试菌株）最有效的化合物。此外，微量稀释法，在广泛的面板中的微生物进行评估，发现李斯特菌和金黄色葡萄球菌作为最敏感的人类测试菌株和Clavibacter密歇根州之间的植物病原体。GC-MS分析结果表明，活性成分仅占总EO的一小部分，主要成分为大根香叶烯D（23.16%）、β-香芹烯（15.53%）、δ-杜松烯（5.50%）、α-可巴烯（5.08%）和α-水芹烯（3.48%），为该属植物中罕见的成分。最后，H。检查sprucei EO对A549的细胞毒性潜力（肺癌）和MCF-7（乳腺癌）细胞系在48小时后显示出针对两种细胞系的有希望的细胞毒性作用（IC50 A549 = 44.05 ± 2.35 µg/mL; IC50 MCF-7 = 32.76 ± 4.92 µg/mL）和72 h（IC50 A549 = 43.55 ± 2.80 µg/mL; IC50 MCF-7 = 33.64 ± 0.43 µg/mL）。
Bioautography has been used as rapid and easy strategy to detect and identify bioactive fractions/molecules in the never before investigated Hedyosmum sprucei Solms (Chloranthaceae) essential oil (EO). The antioxidant activity, performed through DPPH bioautographic assay and spectrophotometric evaluations (IC50 = 230 ± 10 µg/mL), seemed to be mainly due to α-cadinol and α-muurolol. (HP)TLC bioautography, focused on antimicrobial capacities, pointed out α-cadinol, α-muurolol, τ-muurolol, caryophyllene oxide, and methyleugenol as the most effective compounds against Staphylococcus aureus, considered as testing strain. Moreover, the microdilution method, assessed among a wide panel of microorganisms, revealed Listeria grayi and Staphylococcus aureus as the most sensitive among human tested strains and Clavibacter michiganensis among phytopathogens. GC-MS chemical profile showed that bioactive molecules represented only a small quantity of the whole EO: germacrene D (23.16%), β-caryophyllene (15.53%), δ-cadinene (5.50%), α-copaene (5.08%), and α-phellandrene (3.48%) were the main compounds, highlighting an uncommon composition among the genus Hedyosmum. Finally, H. sprucei EO was checked for cytotoxic potential against A549 (lung cancer) and MCF-7 (breast cancer) cell lines showing promising cytotoxic effects against both cell lines after 48 h (IC50 A549 = 44.05 ± 2.35 µg/mL; IC50 MCF-7 = 32.76 ± 4.92 µg/mL) and 72 h (IC50 A549 = 43.55 ± 2.80 µg/mL; IC50 MCF-7 = 33.64 ± 0.43 µg/mL).