Sheet-scanned dual-axis confocal microscopy using Richardson-Lucy deconvolution.
Sheet-scanned dual-axis confocal microscopy using Richardson-Lucy deconvolution.
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DOI：
10.1364/ol.39.005431
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发表时间：
2014-09-15
期刊：
影响因子：
3.6
通讯作者：
Liu JT
中科院分区：
文献类型：
--
作者：
Wang D;Meza D;Wang Y;Gao L;Liu JT
我们之前开发了一种线扫描双轴共聚焦（LS-DAC）显微镜，具有亚细胞分辨率，适用于浅深度的高帧率诊断成像。由于沿一维共聚焦的损失，与点扫描DAC显微镜相比，LS-DAC显微镜的对比度（信号与背景比）恶化。然而，通过使用sCMOS相机进行检测，在每个扫描位置成像一个短的倾斜光片。因此，通过仅在一个维度上扫描光片，就可以对薄的3D体进行成像。在薄图像体上进行连续的二维反褶积和三维反褶积，以提高在体积中心的一个面共聚焦图像切片的分辨率和对比度，我们称之为薄片扫描双轴共聚焦（SS-DAC）显微镜。
We have previously developed a line-scanned dual-axis confocal (LS-DAC) microscope with subcellular resolution suitable for high-frame-rate diagnostic imaging at shallow depths. Due to the loss of confocality along one dimension, the contrast (signal-to-background ratio) of a LS-DAC microscope is deteriorated compared to a point-scanned DAC microscope. However, by using a sCMOS camera for detection, a short oblique light-sheet is imaged at each scanned position. Therefore, by scanning the light sheet in only one dimension, a thin 3D volume is imaged. Both sequential two-dimensional deconvolution and three-dimensional deconvolution are performed on the thin image volume to improve the resolution and contrast of one en face confocal image section at the center of the volume, a technique we call sheet-scanned dual-axis confocal (SS-DAC) microscopy.