Novel antifouling and antimicrobial compound from a marine-derived fungus Ampelomyces sp.
Novel antifouling and antimicrobial compound from a marine-derived fungus Ampelomyces sp.
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DOI：
10.1007/s10126-005-6146-2
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发表时间：
2006-11-01
影响因子：
3
通讯作者：
Qian, Pei Yuan
中科院分区：
文献类型：
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作者：
Kwong, Theresa Fuk Ning;Miao, Li;Qian, Pei Yuan
在这项研究中，使用生物测定指导的分离和纯化程序，我们获得了3-氯-2，5-二羟基苯甲醇从海洋来源的Ampelomyces物种，有效地抑制幼虫定居的管虫Hydroides elegans和cyprids的藤壶藤壶amphitrite。3-氯-2，5-二羟基苯甲醇对幼虫的抑制作用是无毒的，其EC_（50）为3.19 ~ 3.81 μ g/ml，而对B的LC_（50）为266.68 λ g/ml。对双沟藻的EC_（50）为0.67 μ g/ml ~ 0.78 μ g/ml，LC_（50）为2.64 μ g/ml。表明该化合物的抑制作用是无毒的。在50 μ g/片的浓度下，该化合物对在圆盘扩散生物测定中测试的15种海洋细菌中的13种的生长显示出强烈的抑制作用。总体而言，该化合物对细菌和幼虫定居的高抑制活性以及对藤壶和多毛类幼虫的无毒或低毒性表明该化合物可能是一种有效的防污剂和/或抗生素。
In this study, using a bioassay-guided isolation and purification procedure, we obtained 3-chloro-2,5-dihydroxybenzyl alcohol from a marine-derived Ampelomyces species that effectively inhibited larval settlement of the tubeworm Hydroides elegans and of cyprids of the barnacle Balanus amphitrite. The inhibitive effect on larval settlement was nontoxic and the EC50 of 3-chloro-2,5-dihydroxybenzyl alcohol ranged from 3.19 mu g ml(-1) to 3.81 mu g ml(-1) while the LC50 was 266.68 lambda g ml(-1) for B. amphitrite cyprids; EC50 ranged from 0.67 mu g ml(-1) to 0.78 mu g ml(-1), and LC50 was 2.64 mu g ml(-1) for competent larvae of H. elegans, indicating that inhibitive effect of this compound was nontoxic. At a concentration of 50 mu g per disc, this compound showed strong inhibitive effects on the growth of 13 out of 15 marine bacterial species tested in disc diffusion bioassay. Overall, the high inhibitory activities against bacteria and larval settlement as well as the non- or low-toxic nature of this compound to the barnacle and polychaete larvae suggest this compound could be a potent antifoulant and/or antibiotic.