Dielectrophoresis in a Slanted Microchannel for Separation of Microparticles and Bacteria
Dielectrophoresis in a Slanted Microchannel for Separation of Microparticles and Bacteria
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DOI：
10.1166/jnn.2013.8158
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发表时间：
2013-12-01
影响因子：
--
通讯作者：
Park, Sungsu
中科院分区：
文献类型：
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作者：
Nam, Seong-Won;Kim, So Hyun;Park, Sungsu
介质电泳（DEP）是捕获、处理和分离各种介电粒子的有效方法。为了产生DEP力，由电极阵列产生空间非均匀电场，而无电极DEP是通过在两个电极之间放置绝缘材料来实现的。在这里，我们描述了一种利用倾斜微通道产生非均匀电场的新DEP方法。电场梯度是由通道中的斜率引起的，可以用来移动和分离粒子。基于微通道三维结构所产生的逐渐电场，我们的方法可以实现不同尺寸的颗粒仅通过DEP力分离而不流动。斜微通道是用商用紫外光固化光聚合物（noa63）通过复制成型技术制备的，并作为两个铟锡氧化物膜之间的绝缘层粘合。通过施加电场，不同大小（直径6-45 μ m）的聚苯乙烯珠根据施加的电场强度和频率被捕获和分离。利用该方法，在倾斜微通道中成功分离了附着在抗体偶联微珠上的条件致病菌铜绿假单胞菌和大肠杆菌。
Dielectrophoresis (DEP) is an effective method to trap, manipulate and separate various dielectric particles. To generate a DEP force, a spatially nonuniform electrical field has been generated by an array of electrodes, while electrodeless DEP has been accomplished by placing an insulating material between two electrodes. Here, we describe a new DEP method for generating a nonuniform electrical field using a slanted microchannel. The electric field gradient is induced due to a slope in the channel and can be used to move and separate particles. Based on the gradual electric field induced by three dimensional structure of the microchannel, our method enables particles of different sizes to be separated solely by DEP force without flow. The slanted microchannel was easily fabricated by a replica molding technique using a commercial UV-cured photopolymer (NOA 63) and bonded as an insulating layer between two indium-tin-oxide films. By applying the electrical field, polystyrene beads of different sizes (6-45 mu m in diameter) were trapped and separated depending on the applied electric strength and frequency. Using this method, the opportunistic pathogen Pseudomonas aeruginosa attached to antibody-conjugated microbeads was successfully separated from Escherichia coli in a slanted microchannel.