The Excitatory Neuronal Network of the C2 Barrel Column in Mouse Primary Somatosensory Cortex
The Excitatory Neuronal Network of the C2 Barrel Column in Mouse Primary Somatosensory Cortex
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DOI：
10.1016/j.neuron.2008.12.020
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发表时间：
2009-01-29
期刊：
影响因子：
16.2
通讯作者：
Petersen, Carl C. H.
中科院分区：
文献类型：
--
作者：
Lefort, Sandrine;Tomm, Christian;Petersen, Carl C. H.
新皮质柱内的局部微电路构成了感觉处理的关键决定因素。在这里，我们研究了一个解剖学上定义的皮质柱，小鼠初级体感皮质的C2桶柱的兴奋性突触神经元网络。众所周知，这个皮质柱处理与C2胡须相关的触觉信息。通过多个同步的全细胞记录，我们量化了位于C2桶柱所有皮质层的各个兴奋性神经元之间的连接图。突触的连通性强烈依赖于突触前和突触后神经元的体层位置，为层特异性信号通路提供了确凿的证据。突触前4层神经元对皮质柱的兴奋性影响最强。我们在所有层都发现了罕见的大幅度突触连接，这可能对可靠的信息处理有很大贡献。我们的数据集首次在单细胞分辨率下提供了生理上相关和解剖学上定义良好的皮质柱的兴奋性突触连线图的功能描述。
Local microcircuits within neocortical columns form key determinants of sensory processing. Here, we investigate the excitatory synaptic neuronal network of an anatomically defined cortical column, the C2 barrel column of mouse primary somatosensory cortex. This cortical column is known to process tactile information related to the C2 whisker. Through multiple simultaneous whole-cell recordings, we quantify connectivity maps between individual excitatory neurons located across all cortical layers of the C2 barrel column. Synaptic connectivity depended strongly upon somatic laminar location of both presynaptic and postsynaptic neurons, providing definitive evidence for layer-specific signaling pathways. The strongest excitatory influence upon the cortical column was provided by presynaptic layer 4 neurons. In all layers we found rare large-amplitude synaptic connections, which are likely to contribute strongly to reliable information processing. Our data set provides the first functional description of the excitatory synaptic wiring diagram of a physiologically relevant and anatomically well-defined cortical column at single-cell resolution.