Low diversity of the gut microbiota in infants with atopic eczema
Low diversity of the gut microbiota in infants with atopic eczema
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DOI：
10.1016/j.jaci.2011.10.025
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发表时间：
2012-02-01
影响因子：
14.2
通讯作者：
Jenmalm, Maria C.
中科院分区：
文献类型：
--
作者：
Abrahamsson, Thomas R.;Jakobsson, Hedvig E.;Jenmalm, Maria C.
背景资料：对于过敏性疾病发病率的增加，儿童早期肠道微生物群的低总多样性是否比特定细菌物种的流行率改变更重要，这是有争议的。强大的、无需培养的分子方法的出现使得有可能在大型队列中表征总微生物组，直至属水平。目的：我们试图评估微生物多样性，并表征与特应性湿疹发展相关的第一年粪便中的优势细菌。方法：用条形码16 S rDNA 454-焦磷酸测序法分析了1周、1个月、20例IgE相关性湿疹婴儿和20例2岁以下无任何过敏表现的婴儿（ClinicalTrials.gov ID NCT 01285830）。结果：患有IgE相关湿疹的婴儿在1个月时总微生物群的多样性较低（P = .004），1个月时细菌拟杆菌门和拟杆菌属的多样性较低（P = .02和P = .01）和变形菌门在12个月大（P = .02）。1个月龄时的微生物群比12个月龄时的微生物群更不均匀，个体间差异很大。在12个月时，当微生物群已经稳定时，包括革兰氏阴性生物体的变形菌在没有过敏表现的婴儿中更丰富（R [edgeR]检验中的数字基因表达的经验分析：P = 0.008，q = 0.02）。（J Allergy Clin Immunol 2012;129：434-40.）
Background: It is debated whether a low total diversity of the gut microbiota in early childhood is more important than an altered prevalence of particular bacterial species for the increasing incidence of allergic disease. The advent of powerful, cultivation-free molecular methods makes it possible to characterize the total microbiome down to the genus level in large cohorts.Objective: We sought to assess microbial diversity and characterize the dominant bacteria in stool during the first year of life in relation to atopic eczema development.Methods: Microbial diversity and composition were analyzed with barcoded 16S rDNA 454-pyrosequencing in stool samples at 1 week, 1 month, and 12 months of age in 20 infants with IgE-associated eczema and 20 infants without any allergic manifestation until 2 years of age (ClinicalTrials.gov ID NCT01285830).Results: Infants with IgE-associated eczema had a lower diversity of the total microbiota at 1 month (P = .004) and a lower diversity of the bacterial phylum Bacteroidetes and the genus Bacteroides at 1 month (P = .02 and P = .01) and the phylum Proteobacteria at 12 months of age (P = .02). The microbiota was less uniform at 1 month than at 12 months of age, with a high interindividual variability. At 12 months, when the microbiota had stabilized, Proteobacteria, comprising gram-negative organisms, were more abundant in infants without allergic manifestation (Empirical Analysis of Digital Gene Expression in R [edgeR] test: P = .008, q = 0.02).Conclusion: Low intestinal microbial diversity during the first month of life was associated with subsequent atopic eczema. (J Allergy Clin Immunol 2012;129:434-40.)