EXPRESSION OF 2 ZEBRAFISH ORTHODENTICLE-RELATED GENES IN THE EMBRYONIC BRAIN
EXPRESSION OF 2 ZEBRAFISH ORTHODENTICLE-RELATED GENES IN THE EMBRYONIC BRAIN
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DOI：
10.1016/0925-4773(94)90062-0
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发表时间：
1994-12-01
影响因子：
2.6
通讯作者：
WEINBERG, ES
中科院分区：
文献类型：
--
作者：
LI, YB;ALLENDE, ML;WEINBERG, ES
为了分析斑马鱼胚胎最前端区花纹形成的分子机制，我们分离了两个斑马鱼正畸齿（Drosophila orthodenticle, otd）和两个小鼠Otx基因相关的zOtx1和zOtx2序列。zOtx1和zOtx2编码的预测基因产物与相应小鼠蛋白的一致性分别为82%和94%。这两种斑马鱼基因的转录本突然高水平地出现在动物中原肠胚极的一个三角形斑块上，这个区域包含了注定要成为中脑和前脑的细胞。在发育9到14小时之间，zOtx转录本以每种基因特有的方式从前脑区域消失，从14到24小时，前脑和中脑的特定区域表达一种或两种基因。在10-30小时胚胎中，这两个基因的表达后限在中脑后缘形成一个尖锐的边界。与小鼠一样，zOtx基因的早期表达模式与确定中脑和前脑区域的作用一致。然而，在表达这两个物种的基因的前脑和中脑区域之间存在许多有趣的差异。
To analyze the molecular mechanism of pattern formation in the anteriormost regions of the zebrafish embryo, we isolated two zebrafish sequences, zOtx1 and zOtx2, related to the Drosophila orthodenticle (otd) and two murine Otx genes. zOtx1 and zOtx2 encode predicted gene products which are 82% and 94% identical to the corresponding mouse proteins. Transcripts of both zebrafish genes appear abruptly at high levels in a triangular patch at the animal pole of the mid-gastrula, a region which contains cells fated to become midbrain and forebrain. Between 9 and 14 h of development, zOtx transcripts disappear from forebrain regions in a manner characteristic for each gene, and from 14 to 24 h, particular regions of the forebrain and midbrain express one or both genes. The posterior limit of expression of both genes in 10-30-h embryos forms a sharp boundary at the posterior border of the midbrain. As in the mouse, the early expression patterns of the zOtx genes are consistent with a role in defining mid-brain and forebrain territories. However, there are a number of interesting differences between the forebrain and midbrain regions which express the genes in the two species.