Three genes for the elongation factor EF-1 alpha in Mucor racemosus.
Three genes for the elongation factor EF-1 alpha in Mucor racemosus.
复制标题
总状毛霉中延伸因子 EF-1 α 的三个基因。
DOI：
10.1128/mcb.6.2.593-600.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
Sypherd,PS
中科院分区：
文献类型：
--
作者：
Linz,JE;Katayama,C;Sypherd,PS
从总状毛霉中克隆了3个编码蛋白合成延伸因子1α (EF-1α)的基因。通过杂交选择翻译鉴定了一个110碱基对（bp）的ef -1α-特异性cDNA克隆。该cDNA的核苷酸序列与酿酒糖酵母菌EF-1α （tef1和tef2）的一个区域具有显著的同源性。该cDNA用于分离含有EF-1α基因部分的850-bpEcoRI基因组DNA片段。筛选a λ/M。用850-bpEcoRI探针在总状花序基因组DNA库中鉴定出三个DNA片段，在一个短重叠区域内含有一个共同的850-bpEcoRI片段。3个EF-1α DNA片段的S1核酸酶分析表明，EF-1α转录物覆盖了克隆中的短重叠区。从每个克隆的侧翼区域纯化的限制性内切片段用于检测m的aHindIII酶切。racemosusgenomic DNA。每个侧翼探针与三个DNA片段中的一个杂交，这些DNA片段杂交成850-bp的ef -1α-特异性探针。其中一个基因的两个随机“霰弹枪克隆”的核苷酸序列数据显示，与s的两个区域具有良好的同源性。酵母TEF1。这些数据表明EF-1α在m中存在三个基因。总状花序位于基因组的独特位置。
We cloned three genes from Mucor racemosus coding for protein synthesis elongation factor 1α (EF-1α). A 110-base-pair (bp) EF-1α-specific cDNA clone was identified by hybrid-selected translation. The nucleotide sequence of the cDNA showed significant homology to a region of theSaccharomyces cerevisiaegenes for EF-1α (TEF1andTEF2). The cDNA was used to isolate an 850-bpEcoRI genomic DNA fragment containing a portion of the EF-1α gene. Screening of a λ/M.racemosusgenomic DNA bank with the 850-bpEcoRI probe resulted in the identification of three DNA fragments containing a common 850-bpEcoRI fragment within a short overlapping region. S1 nuclease analysis of the three EF-1α DNA fragments showed that the EF-1α transcript covered the short overlapping region in the clones. Restriction fragments purified from flanking regions in each clone were used to probe aHindIII digest ofM. racemosusgenomic DNA. Each flanking probe hybridized to one of three DNA fragments which hybridized to the 850-bp EF-1α-specific probe. Nucleotide sequence data from two random “shotgun clones” of one of the three genes show good homology to two regions ofS. cerevisiae TEF1. The data indicate the presence of three genes for EF-1α inM. racemosuslocated at unique sites in the genome.