Characteristics of a rat model of an open craniocerebral injury at simulated high altitude.
Characteristics of a rat model of an open craniocerebral injury at simulated high altitude.
复制标题
模拟高海拔开放性颅脑损伤大鼠模型的特点
DOI：
10.1097/wnr.0000000000000259
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发表时间：
2014-11-12
期刊：
影响因子：
1.7
通讯作者：
Feng H
中科院分区：
文献类型：
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作者：
Yu AY;Xu QH;Hu SL;Li F;Chen YJ;Yin Y;Zhu G;Lin JK;Feng H
目的建立模拟高原开放性颅脑损伤大鼠模型，探讨该模型的特点。将大鼠随机分为常压组、高原组及相应的对照组。采用射钉法建立大鼠开放性颅脑损伤模型。模拟高海拔条件下建立了一个低压舱在0.6 ATA模拟压力在海拔4000米。观察各组死亡率、脑含水量（BWC）、伊文思蓝含量、病理学改变、局部脑血流量（rCBF）、脑组织氧分压（PbtO2）、脑干诱发电位等指标。伤后72h内高原组死亡率明显高于常压组（P<0.05）。伤后48h BWC和伊文思蓝含量增加（P<0.05），脑组织病理改变较伤后严重。而rCBF和PbtO2在72 h时明显降低（P<0.01），脑干诱发电位值明显延长（P<0.05）。rCBF与BWC呈负相关，与PbtO2呈正相关。采用射钉法和低压舱法可成功建立模拟高原开放性颅脑损伤大鼠模型。高原组的损伤特征较常压组严重、迅速、持续时间长。
To establish a rat model of an open craniocerebral injury at simulated high altitude and to examine the characteristics of this model. Rats were divided randomly into a normobaric group and a high-altitude group and their corresponding control groups. A rat model of an open craniocerebral injury was established with a nail gun shot. Simulated high-altitude conditions were established with a hypobaric chamber at 0.6 ATA to mimic pressure at an altitude of 4000 m. Mortality, brain water content (BWC), Evans blue content, pathology, regional cerebral blood flow (rCBF), partial pressure of brain tissue oxygen (PbtO2), and brainstem auditory-evoked potential were observed after injury. The mortality of the high-altitude group was significantly greater than that of the normobaric group within 72 h after injury (P<0.05). BWC and Evans blue content increased by 48 h after injury (P<0.05); pathological changes in damaged brains were more serious. In contrast, rCBF and PbtO2 had decreased markedly by 72 h (P<0.01); brainstem auditory-evoked potential values were significantly prolonged (P<0.05). Moreover, an inverse correlation between rCBF and BWC and a positive correlation between rCBF and PbtO2 were found. The rat model of an open craniocerebral injury at simulated high altitude can be established successfully using a nail gun shot and a hypobaric chamber. The injury characteristics at high altitude were more serious, rapid, and prolonged than those in the normobaric group.
影响因子：
--
作者：
Wani AA;Ramzan AU;Dar TI;Malik NK;Khan AQ;Wani MA;Alam S;Nizami FA
通讯作者：
Nizami FA