Defining embryonic stem cell identity using differentiation-related microRNAs and their potential targets
Defining embryonic stem cell identity using differentiation-related microRNAs and their potential targets
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DOI：
10.1007/s00335-007-9032-6
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发表时间：
2007-05-01
期刊：
影响因子：
2.5
通讯作者：
Strauss, William M.
中科院分区：
文献类型：
--
作者：
Chen, Caifu;Ridzon, Dana;Strauss, William M.
胚胎干细胞（ES细胞）的定量分子鉴定是了解其功能特性的先决条件。关于microRNA（miRNAs）在ES细胞身份调节中的作用知之甚少。miRNA表达的统计分析揭示了独特的表达特征，可以明确分类小鼠ES（mES），胚状体（mEB），和体细胞组织。对这些数据集的分析也进一步证实了小鼠发育过程中miRNA的非限制性表达。使用miRNAs和mRNAs的组合全基因组表达分析，我们观察到miRNAs与其预测靶点之间的基因表达存在负相关和正相关。ES特异性miRNAs与其预测的靶点呈正相关，提示在ES维持或分化过程中，mES特异性miRNAs可能具有不同的作用或机制。细胞身份的概念随着技术的发展而改变，本研究通过已知维数的通用统计方法重新定义了细胞身份。
Defining the identity of embryonic stem (ES) cells in quantitative molecular terms is a prerequisite to understanding their functional characteristics. Little is known about the role of microRNAs (miRNAs) in the regulation of ES cell identity. Statistical analysis of miRNA expression revealed unique expression signatures that could definitively classify mouse ES (mES), embryoid bodies (mEB), and somatic tissues. Analysis of these data sets also provides further confirmation of the nonrestrictive expression of miRNAs during murine development. Using combined genome-wide expression analyses of both miRNAs and mRNAs, we observed both negative and positive correlations in gene expression between miRNAs and their predicted targets. ES-specific miRNAs were positively correlated with their predicted targets, suggesting that mES-specific miRNAs may have a different role or mechanism in regulating their targets in mES maintenance or differentiation. The concept of cellular identity has changed with technology; this study redefines cellular identity by a generic statistical method of known dimension.