IGM MOLECULES WITH AND WITHOUT J-CHAIN IN SERUM AND AFTER PURIFICATION, STUDIED BY ULTRACENTRIFUGATION, ELECTROPHORESIS, AND ELECTRON-MICROSCOPY
IGM MOLECULES WITH AND WITHOUT J-CHAIN IN SERUM AND AFTER PURIFICATION, STUDIED BY ULTRACENTRIFUGATION, ELECTROPHORESIS, AND ELECTRON-MICROSCOPY
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DOI：
10.1111/j.1365-3083.1975.tb02620.x
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发表时间：
1975-01-01
影响因子：
3.7
通讯作者：
CHRISTENSEN, TB
中科院分区：
文献类型：
--
作者：
ESKELAND, T;CHRISTENSEN, TB
本文用分析性超离心、速率区向超离心对3例Waldenström巨球蛋白血症患者血清中纯IgM制剂和相应血清中的IgM分子进行了研究。琼脂糖-聚丙烯酰胺凝胶电泳和电子显微镜。其中一种IgM制剂不含J链，另外两种含有J链。实验表明，含J链的IgM由五聚体分子组成，而不含J链的IgM制备中主要分子为六聚体，但也有五聚体、四聚体，甚至更小的分子。在这两种类型的IgM中，分子形成了广泛的聚集体，在纯化的IgM中通常比在血清中更丰富。有些聚集体仅靠非共价力保持在一起。
IgM molecules in pure IgM preparations and in the corresponding sera from three patients with Waldenström's macroglobulinemia have been studied by analytical ultracentrifugation, rate zonal ultratcntrifugation. agarose‐polyacrylamide gel electrophoresis, and electron microscopy. One of the IgM preparations was without J chain, while the two others contained J chain. The experiments revealed that IgM with J chain consisted of pentameric molecules: whereas in IgM preparations without J chain the dominating molecules were hexamers, but also pentamers, tetramers, and even smaller molecules were found. In both types of IgM the molecules formed a wide range of aggregates, which were usually more abundant in the purified IgM than in serum. Some of the aggregates were kept together by noncovalent forces only.