Immunolocalization of IL-17A, IL-17B, and their receptors in chondrocytes during fracture healing
Immunolocalization of IL-17A, IL-17B, and their receptors in chondrocytes during fracture healing
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DOI：
10.1369/jhc.7a7223.2007
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发表时间：
2008-02-01
影响因子：
3.2
通讯作者：
Reddi, A. Hari
中科院分区：
文献类型：
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作者：
Kokubu, Takeshi;Haudenschild, Dominik R.;Reddi, A. Hari
长骨的骨折愈合是止血、短暂炎症、祖细胞趋化、有丝分裂、软骨分化和骨替代的连续多步骤级联。这种多步骤级联反应是由细胞因子和形态发生素协调的。白细胞介素（IL）-17家族的成员，包括IL-17 B，已被确定在软骨中，但其在骨折愈合过程中的表达是未知的。在这项研究中，我们确定了免疫定位的细胞因子IL-17 A和IL-17 B，沿着IL-17受体（IL-17 R）和IL-17受体样蛋白（IL-17 RL），在一个标准模型中的骨折修复序列。结果被扩展到长骨的骨骺生长板的发育变化。IL-17家族成员定位于骨折骨痂中的软骨细胞中。此外，我们发现这些细胞因子及其受体在软骨细胞中的定位在骺生长板的软骨内分化程序中的显着的相似之处。
Fracture healing in long bones is a sequential multistep cascade of hemostasis, transient inflammation, chemotaxis of progenitor cells, mitosis, differentiation of cartilage, and replacement with bone. This multistep cascade is orchestrated by cytokines and morphogens. Members of the interleukin (IL)-17 family, including IL-17B, have been identified in cartilage, but their expression during fracture healing is unknown. In this study, we determined the immunolocalization of cytokines IL-17A and IL-17B, along with the IL-17 receptor (IL-17R) and IL-17 receptor-like protein (IL-17RL), during the sequence of fracture repair in a standard model. The results were extended to developmental changes in the epiphyseal growth plate of long bones. Members of the IL-17 family were localized in chondrocytes in the fracture callus. Moreover, we found significant parallels to the localization of these cytokines and their receptors in chondrocytes during an endochondral differentiation program in the epiphyseal growth plate.