Distribution and physiological effect of enterin neuropeptides in the olfactory centers of the terrestrial slug Limax.
Distribution and physiological effect of enterin neuropeptides in the olfactory centers of the terrestrial slug Limax.
复制标题
陆生蛞蝓嗅觉中枢肠神经肽的分布及其生理效应。
DOI：
10.1007/s00359-020-01400-2
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发表时间：
2020
期刊：
影响因子：
2.1
通讯作者：
and Matsuo Y
中科院分区：
文献类型：
--
作者：
*Matsuo R;Kobayashi S;Furuta A;Osugi T;Takahashi T;Satake H;and Matsuo Y
在腹足动物中，神经肽的功能主要是在外周运动系统中进行的研究。它们在中枢神经系统中的功能作用受到的关注较少。前脑是陆栖鼻涕虫的次级嗅觉中枢，由几十万个中间神经元组成，在嗅觉学习和记忆中起着关键作用。在目前的研究中，我们发现肠蛋白是一种肌动蛋白，在海兔的肠道和血管系统中发挥作用，在利马斯湖的前脑中也有表达。这些表达肠素的神经元主要在前脑的细胞层内进行投射。外源性应用肠肽可降低前脑局部场电位的振荡频率。初级嗅觉中枢触角神经节的局部场电位振荡也受到肠肽的调制。全细胞膜片钳记录显示，前脑的调制作用是由于肠肽对爆裂神经元的抑制作用，而这些神经元是决定前脑振荡活动的核心。我们的结果揭示了肌动神经肽肠素在陆栖腹足类高级嗅觉功能中的新作用。
In gastropods, the function of neuropeptides has been studied primarily in the peripheral motor systems. Their functional roles in the central nervous system have received less attention. The procerebrum, the secondary olfactory center of the terrestrial slugLimax, consists of several hundred thousand interneurons, and plays a pivotal role in olfactory learning and memory. In the present study, we found that enterin, known as a myoactive peptide functioning in the enteric and vascular system ofAplysia, is expressed in the procerebrum ofLimax. These enterin-expressing neurons primarily make projections within the cell mass layer of the procerebrum. The oscillatory frequency of the local field potential in the procerebrum was reduced by an exogenous application of enterin. The local field potential oscillation in the tentacular ganglion, the primary olfactory center, was also modulated by enterin. Whole-cell patch-clamp recordings revealed that the modulatory effect in the procerebrum was due to the inhibitory effect of enterin on the bursting neurons, which function as the kernels determining the oscillatory activity of the procerebrum. Our results revealed the novel role of the myoactive neuropeptide enterin in the higher olfactory function in terrestrial gastropods.