Fibrillin-rich microfibrils are reduced in photoaged skin. Distribution at the dermal-epidermal junction
Fibrillin-rich microfibrils are reduced in photoaged skin. Distribution at the dermal-epidermal junction
复制标题
DOI：
10.1046/j.1523-1747.1999.00562.x
复制
发表时间：
1999-05-01
影响因子：
6.5
通讯作者：
Kielty, CM
中科院分区：
文献类型：
--
作者：
Watson, REB;Griffiths, CEM;Kielty, CM
长期暴露在阳光下会导致皮肤光老化，出现深深的粗皱纹并失去弹性。我们研究了光老化和光保护皮肤中富含原纤维蛋白的微原纤维（弹性纤维网络的关键结构成分）的分布和丰度。对 16 名具有临床光老化范围的受试者的光老化前臂、光保护髋部和上臂内臂进行穿刺活检，检查原纤维蛋白 1 和原纤维蛋白 2 的表达以及微纤维分布。原位杂交显示，在严重光老化的前臂活检中，相对于光保护的真皮位点，fibrillin-1 mRNA 水平降低，但 fibrillin-2 mRNA 水平不变。免疫组织化学方法表明，中度至重度光老化的前臂皮肤真皮-表皮交界处的微纤维显着减少。共聚焦显微镜显示，光老化皮肤中的乳头状真皮微纤维网络被截断和耗尽。这些研究强调，与上层真皮相关的富含原纤维蛋白的微原纤维网络在太阳照射后经历了广泛的重塑。这些变化可能导致光老化的临床特征，例如皱纹形成和弹性丧失。
Chronic sun exposure results in photoaged skin with deep coarse wrinkles and loss of elasticity. We have examined the distribution and abundance of fibrillin-rich microfibrils, key structural components of the elastic fiber network, in photoaged and photoprotected skin. Punch biopsies taken from photoaged forearm and from photoprotected hip and upper inner arm of 16 subjects with a clinical range of photoaging were examined for fibrillin-1 and fibrillin-2 expression and microfibril distribution. lit situ hybridization revealed decreased fibrillin-1 mRNA but unchanged fibrillin-2 mRNA levels in severely photoaged forearm biopsies relative to photoprotected dermal sites. An immunohistochemical approach demonstrated that microfibrils at the dermal-epidermal junction were significantly reduced in moderate to severely photoaged forearm skin. Confocal microscopy revealed that the papillary dermal microfibrillar network was truncated and depleted in photoaged skin. These studies highlight that the fibrillin-rich microfibrillar network associated with the upper dermis undergo es extensive remodeling following solar irradiation. These changes may contribute to the clinical features of photoaging, such as wrinkle formation and loss of elasticity.