Encapsulation of Curcumin Nanoparticles with MMP9-Responsive and Thermos-Sensitive Hydrogel Improves Diabetic Wound Healing
Encapsulation of Curcumin Nanoparticles with MMP9-Responsive and Thermos-Sensitive Hydrogel Improves Diabetic Wound Healing
复制标题
用 MMP9 响应性和热敏性水凝胶封装姜黄素纳米颗粒可改善糖尿病伤口愈合
DOI：
10.1021/acsami.8b03868
复制
发表时间：
2018-05-16
影响因子：
9.5
通讯作者：
Wang, Yunfang
中科院分区：
文献类型：
--
作者：
Liu, Juan;Chen, Zhiqiang;Wang, Yunfang
糖尿病患者伤口愈合受损通常会导致危及生命的并发症。为了开发一种用于在糖尿病患者中有效和安全地加速皮肤伤口愈合的系统，设计了以明胶微球（GM）形式负载的含有纳米药物的热敏水凝胶，以递送姜黄素（Cur）作为治疗药物。Cur是一种天然存在的多酚化合物，具有广泛的生物学功能，可用于潜在的治疗。由于Cur分子在生物利用度和体内稳定性方面都较弱，因此Cur的递送需要其他分子的辅助，以持续的方式作为载体媒介物用于治疗用途。首先，进行了Cur纳米颗粒（CNPs）的自组装以提高生物利用度。CNP进一步被封闭到GM中，用于响应通常在糖尿病不愈合伤口部位过度表达的基质金属蛋白酶（MMPs）。将含有CNP的GM负载到温敏性水凝胶中，并最终证明了在伤口床处特异性诱导药物释放的能力，这促进了链脲佐菌素诱导的糖尿病小鼠标准化皮肤伤口的愈合功效。我们的研究结果表明，成功开发的CNP递送系统具有显著促进皮肤伤口愈合的能力，这表明它有可能成为具有抗氧化剂和促进细胞迁移特性的伤口敷料。
Impaired wound healing in diabetics usually leads to life-threatening complications. To develop a system for fastening skin wound healing efficiently and safely in diabetics, thermos sensitive hydrogel containing the nanodrug, loaded in the form of gelatin microspheres (GMs), was designed to deliver curcumin (Cur) as a therapeutic drug. Cur is a naturally existing polyphenolic compound with a broad range of biological functions useful for potential therapies. Because Cur molecule has weakness in both bioavailability and in vivo stability, delivery of Cur requires assistance from other molecules to act as carrier vehicles in a sustained manner for therapeutic use. At first, self-assembly of Cur nanoparticles (CNPs) was done to improve bioavailability. The CNPs were further enclosed into GMs for responding to the matrix metalloproteinases (MMPs) that usually overexpress at diabetic nonhealing wound sites. The GMs containing CNPs were loaded into the thermos-sensitive hydrogel and were finally proved for the capacity of specially induced drug release at the wound bed, which promoted the efficacy in healing the standardized skin wounds in streptozotocin-induced diabetic mice. Our results indicated that the successfully developed CNP delivery system had the capacity to significantly promote skin wound healing, which suggested that it could have the potential to become a wound dressing with the properties of antioxidants and promotions of cell migration.