The intestinal microflora in allergic Estonian and Swedish 2-year-old children
The intestinal microflora in allergic Estonian and Swedish 2-year-old children
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DOI：
10.1046/j.1365-2222.1999.00560.x
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发表时间：
1999-03-01
影响因子：
6.1
通讯作者：
Mikelsaar, M
中科院分区：
文献类型：
--
作者：
Björkstén, B;Naaber, P;Mikelsaar, M
背景过敏性疾病的患病率似乎在过去35-40年中有所增加。此外，与西欧相比，中欧和东欧前社会主义国家的儿童中过敏性疾病不太常见，已经提出，婴儿期和幼儿期减少的微生物刺激将导致免疫系统的出生后成熟较慢，并在TH 1-和TH 2-之间形成最佳平衡。目的：在两个变态反应流行率低（爱沙尼亚）和高（瑞典）的国家，检验儿童变态反应性疾病可能与其肠道微生物菌群差异有关的假设。它;方法从一项前瞻性研究过敏的发展与环境因素，29爱沙尼亚和33瑞典2岁的儿童被选中。他们要么是非过敏性（n = 36）或确诊过敏（n = 27），通过典型病史和至少一个阳性皮肤点刺试验鸡蛋或牛奶验证。将称重的粪便样品系列稀释（10（-2）-10（-9）），并在无氧条件下进行浓缩。计算每个孩子的各种属和种的数量。结果爱沙尼亚和瑞典的过敏儿童与非过敏儿童相比，乳酸杆菌定植率较低（P < 0.01）。而过敏儿童的需氧微生物计数高于对照组（P < 0.05），尤其是大肠菌群（P < 0.01）和金黄色葡萄球菌（P < 0.05）。过敏儿童肠道植物群中需氧菌的比例也高于正常儿童（P < 0.05），而厌氧菌的比例则相反（P < 0.05）。同样，在过敏的儿童大肠菌群的比例较高（P < 0.05）和拟杆菌低于（P < 0.05）在nonallergic childhood.结论土著肠道植物群的差异可能会影响儿童早期免疫系统的发育和启动，类似于什么已经显示在啮齿类动物。肠道微生物菌群在婴儿免疫力发展中的作用以及在以后的生活中对过敏性疾病的可能后果需要进一步研究，特别是因为它可以很容易地用于干预，作为通过施用益生菌进行过敏的一级预防的手段。
Background The prevalence of allergic diseases seems to have increased particularly over the past 35-40 years. Furthermore, allergic disease is less common among children in the formerly socialist countries of central and Eastern Europe as compared with Western Europe, It has been suggested that a reduced microbial stimulation during infancy and early childhood would result in a slower postnatal maturation of the immune system and development of an optimal balance between TH1- and TH2-like immunity.Aims To test the hypothesis that allergic disease among children may be associated with differences in their intestinal microflora in two countries with a low (Estonia) and a high (Sweden) prevalence of allergy. it;Methods From a prospective study of the development of allergy in relation to environmental factors, 29 Estonian and 33 Swedish 2-year-old children were selected. They were either nonallergic (n = 36) or had a confirmed diagnosis of allergy (n = 27) as verified by typical history and at least one positive skin prick test to egg or cow's milk. Weighed samples of faeces were serially diluted (10(-2)-10(-9)) and frown under amaerobic conditions. The counts of the various genera and species were calculated for each child. In addition, the relative amounts of the particular microbes were expressed as a proportion of the total count.Results The allergic children in Estonia and Sweden were less often colonized with lactobacilli (P < 0.01), as compared with the nonallergic children in the two countries. in contrast, the allergic children harboured higher counts of aerobic micro-organisms (P < 0.05), particularly coliforms (P < 0.01) and Staphylococcus auereus (P < 0.05). The proportions of aerobic bacteria of the intestinal flora were also higher in the allergic children (P < 0.05), while the opposite was true for anaerobes (P < 0.05). Similarly, in the allergic children the proportions of coliforms were higher (P < 0.05) and bacteroides lower (P < 0.05) than in the nonallergic children.Conclusions Differences in the indigenous intestinal flora might affect the development and priming of the immune system in early childhood, similar to what has been shown in rodents. The role of intestinal microflora in relation to the development of infant immunity and the possible consequences for allergic diseases later in life requires further study, particularly as it would be readily available for intervention as a means for primary prevention of allergy by the administration of probiotic bacteria.