Determination of ammonia, ethanol and acetic acid in solution using membrane introduction mass spectrometry.
Determination of ammonia, ethanol and acetic acid in solution using membrane introduction mass spectrometry.
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使用膜引入质谱法测定溶液中的氨、乙醇和乙酸。
DOI：
10.1016/0039-9140(94)80018-9
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Kaiser Re
中科院分区：
文献类型：
--
作者：
Teeter Bk;L. E. Dejarme;Tarun K. Choudhury;R. Cooks;Kaiser Re
方法已经开发，允许应用膜引入质谱（MIMS）监测发酵液的溶液组分使用电子电离。溶液通过流动注射分析（FIA）通过直接插入膜探针输送，该探针在片状结构中装有硅膜。感兴趣的分析物通过膜并被电子植入电离。监测的化合物是氨、乙酸和乙醇，其中氨被检测为一氯胺衍生物，它是在pH为10时加入次氯酸盐后产生的。使用外部标准溶液实现定量。氨的定量动态范围为2 - 8000ppm，乙醇和乙酸的定量动态范围为10 - 1000ppm。该方法提供了对感兴趣的分析物的快速检测，在线监测能力和电子电离的优势。样品导入质谱仪容易、自动，响应时间为几秒，无记忆效应。
Methods have been developed to allow applications of membrane introduction mass spectrometry (MIMS) to monitor solution phase components of fermentation broths using electron ionization. The solutions are transported by flow injection analysis (FIA) through a direct insertion membrane probe, fitted with a silicone membrane in the sheet configuration. Analytes of interest pass through the membrane and are ionized by electron implant ionization. The compounds monitored are ammonia, acetic acid, and ethanol, with ammonia being detected as the monochloramine derivative which is generated at pH 10 upon addition of hypochlorite. Quantitation is achieved using external standard solutions. The dynamic range for the quantification of ammonia is 2–8000 ppm, and for ethanol and acetic acid 10–1000 ppm. This method provides rapid detection of analytes of interest, on-line monitoring capabilities, and the advantage of electron ionization. The introduction of samples into the mass spectrometer is achieved readily and automatically, the response time is a few seconds, and there are no memory effects.