Simultaneous determination of 5-nitroimidazoles and nitrofurans in pork by high-performance liquid chromatography-tandem mass spectrometry.
Simultaneous determination of 5-nitroimidazoles and nitrofurans in pork by high-performance liquid chromatography-tandem mass spectrometry.
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DOI：
10.1016/j.chroma.2008.08.055
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发表时间：
2008-10
期刊：
影响因子：
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通讯作者：
X. Xia;Xiaowei Li;Suxia Zhang;S. Ding;Haiyang Jiang;Jiancheng Li;Jianzhong Shen
中科院分区：
文献类型：
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作者：
X. Xia;Xiaowei Li;Suxia Zhang;S. Ding;Haiyang Jiang;Jiancheng Li;Jianzhong Shen
建立了同时测定猪肉中4种5-硝基咪唑及其两种代谢物和4种硝基呋喃的简便方法。样品的制备过程是基于简单的溶剂萃取/衍生化结合固相萃取（SPE）净化。采用高效液相色谱法（HPLC）和电喷雾电离串联质谱法（ESI/MS/MS）在多反应监测模式下对目标化合物进行鉴定和定量测定。总的校正回收率在93 ~ 106%之间，整个方法的相对标准偏差（rsd）为7 ~ 13%。方法对10种化合物的检出限为0.01 ~ 0.2μg/kg。
A simple and specific method has been developed for the simultaneous determination of four 5-nitroimidazoles and their two corresponding metabolites and four nitrofurans in pork. The sample preparation procedure was based on a simple solvent extraction/derivatization combined with solid-phase extraction (SPE) cleanup. The target compounds were identified and quantitatively determined by high-performance liquid chromatography (HPLC) coupled with electrospray ionization tandem mass spectrometry (ESI/MS/MS) operated in multiple reaction monitoring mode. The overall corrected recoveries ranged between 93 and 106%, with relative standard deviations (RSDs) of 7–13% for the entire procedure. The detection limits of the method for the 10 compounds under study ranged from 0.01 to 0.2μg/kg.