The Use of Real-Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression.
The Use of Real-Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression.
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使用实时逆转录 PCR 评估雌激素受体和雌激素响应基因表达。
DOI：
10.1007/978-1-4939-3127-9_3
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Eyster,KathleenM
中科院分区：
文献类型：
--
作者：
Booze,MichelleL;Eyster,KathleenM
Real-time reverse transcription-polymerase chain reaction (RT-PCR), also known as quantitative RT-PCR (qRT-PCR), is a powerful tool for assessing gene transcription levels. The technique is especially useful for measuring estrogen receptor transcript levels as well as gene expression changes in response to estrogen stimulation as it is quick, accurate, robust, and allows the measurement of gene expression in a variety of tissues and cells. This chapter describes the protocols used for the real-time RT-PCR assay using hydrolysis (TaqMan-type) probes.
DOI：
10.1073/pnas.88.16.7276
发表时间：
1991-08-01
影响因子：
11.1
作者：
HOLLAND, PM;ABRAMSON, RD;GELFAND, DH
通讯作者：
GELFAND, DH
影响因子：
3.1
作者：
Boda, Enrica;Pini, Alessandro;Tempia, Filippo
通讯作者：
Tempia, Filippo