Evaluation of copper availability to plants in copper-contaminated vineyard soils
Evaluation of copper availability to plants in copper-contaminated vineyard soils
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DOI：
10.1016/s0269-7491(00)00067-1
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发表时间：
2001-01-01
影响因子：
8.9
通讯作者：
Pépin, M
中科院分区：
文献类型：
--
作者：
Brun, LA;Maillet, J;Pépin, M
反复使用铜（Cu）杀菌剂来控制葡萄霜霉病，导致葡萄园土壤中Cu的长期积累，现在提出了Cu对各种生物体（包括植物物种）的潜在生物利用度的问题。生物可利用铜可以定义为土壤铜的一部分，可以采取的根，对于一个给定的植物物种。为了评估植物对铜的生物有效性，在温室条件下进行了盆栽试验，在10个葡萄园地块（总铜为38 - 251 mg kg（-1））和两个林地地块（对照土壤，没有收到任何铜的应用;总铜为20-26 mg kg（-1））的上层土层取样的作物品种（玉米）和12个土壤。选择这些土壤是因为它们具有不同的物理（大范围的颗粒大小分布）和化学（从酸性到石灰性土壤）特性。生长35天后，收获植物嫩枝用于分析。将根与土壤颗粒分离以用于进一步分析。然后将玉米根和地上部分中铜的浓度与通过一系列常规提取剂从土壤中提取的铜的量进行比较。在受污染的葡萄园土壤中生长的玉米根中观察到的Cu浓度非常高（在90和600 mg kg（-1）之间），而地上部分的Cu浓度变化很小，保持在较低水平（< 18 mg kg（-1））。玉米根系铜含量随土壤总铜含量的增加而增加，随土壤阳离子交换量的降低而降低。石灰性土壤中玉米根系中铜的积累量可能与酸性土壤中一样高，表明土壤pH值的影响很小。在葡萄园土壤研究的情况下，缺乏相关性发现玉米根和地上部分的铜浓度之间，表明地上部分的分析不会是一个很好的指标植物铜吸收，因为它提供了没有洞察到的真实的量的铜从土壤转移到植物。对于玉米，我们的研究结果表明，萃取与有机络合剂（EDTA，DTPA）和萃取与乙酸铵似乎提供了一个合理的好估计根铜浓度。(C)2000爱思唯尔科技有限公司版权所有。
The repeated use of copper (Cu) fungicides to control vine downy mildew has led to long-term accumulation of Cu in vineyard soils which now raises the issue of the potential bioavailability of Cu for various living organisms including plant species. The bioavailable Cu can be defined as the portion of soil Cu that can be taken up by roots, for a given plant species. In order to evaluate the bioavailability of Cu to plants, a pot experiment was conducted in glasshouse conditions with a crop species (maize) and 12 soils sampled in the upper horizon of 10 vineyard plots (total Cu ranging from 38 to 251 mg kg(-1)) and two woodland plots (control soils that had not received any Cu application; total Cu amounting to 20-26 mg kg(-1)). These soils were selected for their diverse physical (large range of particle size distribution) and chemical (from acid to calcareous soils) properties. After 35 days of growth, plant shoots were harvested for analysis. The roots were separated from soil particles for further analysis. The concentrations of Cu in the roots and aerial parts of the maize were then compared with the amounts of Cu extracted from the soil by a range of conventional extractants. Observed Cu concentrations in maize roots which have grown in contaminated vineyard soils were very high (between 90 and 600 mg kg(-1)), whereas Cu concentrations in the aerial parts varied only slightly and remained low(< 18 mg kg(-1)). Root Cu concentrations observed for maize increased with increasing total Cu content in the soil and with decreasing soil CEC. Cu accumulation in maize roots may be as high in calcareous soils as in acid soils, suggesting that soil pH had little influence. In the case of the Vineyard soils studied, the lack of correlation found for maize between Cu concentrations in roots and in the aerial parts, suggests that an analysis of the aerial parts would not be a good indicator of plant Cu uptake, as it provides no insight into the real amount of Cu transferred from the soil to the plant. For maize, our results show that extraction with organic complexing agents (EDTA, DTPA) and extraction with ammonium acetate seem to provide a reasonably good estimate of root Cu concentration. (C) 2000 Elsevier Science Ltd. All rights reserved.