Successful nodulation of Casuarina by Frankia in axenic conditions
Successful nodulation of Casuarina by Frankia in axenic conditions
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DOI：
10.1111/j.1365-2672.2007.03425.x
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发表时间：
2007-11-01
影响因子：
4
通讯作者：
Prin, Y.
中科院分区：
文献类型：
--
作者：
Echbab, H.;Arahou, M.;Prin, Y.
目的：为了描述导致共生的精细相互作用，需要对共生伙伴进行绝对的微生物控制，即获得体外无菌共生的能力，这是木麻黄-弗兰克氏菌共生体从未满足的条件。研究了无菌培养（不同基质盖管）和非无菌培养（吉布森管、盆栽）对木麻黄生长和根瘤形成的影响。在无菌条件下，C.接种Frankia的Cunninghamiana生长不良，在6周时没有形成结节。在吉布森试管中培养的植株在6周内达到四腋芽期，并在接种后4周形成根瘤。砂盆培养使我们能够将植物的发育阶段与接种acetulation.Conclusions：无菌更换的上限由一个塑料袋增加植物的生长，使根瘤形成接种后6周。新的植物培养系统允许接种后6周形成无菌根瘤。结瘤行为与植物的发育和confination.Significance和影响的研究：这个纯植物的结瘤系统是主要的兴趣在分析Frankia基因在结瘤途径中的作用。
Aims: In order to depict the fine interactions that lead to nodulation, absolute microbiological control of the symbiotic partners is required, i.e. the ability to obtain in vitro axenic nodulation, a condition that has never been fulfilled with the Casuarina-Frankia symbiosis. The effects of culture conditions on plant growth and nodule formation by Casuarina cunninghamiana were investigated.Methods and Results: Axenic (capped tubes with different substrates), and nonaxenic cultures (Gibson tubes, pot cultures) were tested. In axenic conditions, C. cunninghamiana, inoculated with Frankia, had poor growth and did not form nodules at 6 weeks. Plants cultivated in Gibson tubes reached the four axillary shoots stage within 6 weeks and formed nodules 4 weeks after inoculation. Sand-pot cultures allowed us to relate the plant development stage at inoculation with nodulation.Conclusions: The sterile replacement of the cap by a plastic bag increased plant growth and enabled nodule formation 6 weeks after inoculation. The new system of plant culture allows the axenic nodule formation 6 weeks after inoculation. Nodulation behaviour is related to plant development and confinement.Significance and Impact of the Study: This axenic plant nodulation system is of major interest in analysing the roles of Frankia genes in nodulation pathways.