Secondary structure of vertebrate telomerase RNA
Secondary structure of vertebrate telomerase RNA
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DOI：
10.1016/s0092-8674(00)80687-x
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发表时间：
2000-03-03
期刊：
影响因子：
64.5
通讯作者：
Greider, CW
中科院分区：
文献类型：
--
作者：
Chen, JL;Blasco, MA;Greider, CW
端粒酶是一种核糖核蛋白酶，通过在染色体末端添加端粒重复序列来维持端粒长度。端粒酶的基本RNA成分为端粒重复序列的合成提供了模板。为了研究脊椎动物端粒酶RNA的二级结构，从18种哺乳动物、2种鸟类、1种爬行动物、7种两栖动物和4种鱼类中克隆了32个新的端粒酶RNA基因，并进行了序列测定。利用系统发育比较分析，我们提出了一个二级结构，包含四个结构域保守的所有脊椎动物。RNA的十个螺旋区域是普遍保守的，而其他区域在不同种类的脊椎动物之间在长度和序列上有显着差异。提出的脊椎动物端粒酶RNA结构显示出惊人的相似的拓扑结构，以前确定的纤毛虫端粒酶RNA结构，这意味着端粒酶RNA的全球架构的进化保守。
Telomerase is a ribonucleoprotein enzyme that maintains telomere length by adding telomeric sequence repeats onto chromosome ends. The essential RNA component of telomerase provides the template for telomeric repeat synthesis. To determine the secondary structure of vertebrate telomerase RNA, 32 new telomerase RNA genes were cloned and sequenced from a variety of vertebrate species including 18 mammals, 2 birds, 1 reptile, 7 amphibians, and 4 fishes. Using phylogenetic comparative analysis, we propose a secondary structure that contains four structural domains conserved in all vertebrates. Ten helical regions of the RNA are universally conserved while other regions vary significantly in length and sequence between different classes of vertebrates. The proposed vertebrate telomerase RNA structure displays a strikingly similar topology to the previously determined ciliate telomerase RNA structure, implying an evolutionary conservation of the global architecture of telomerase RNA.