Lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels
Lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels
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DOI：
10.1104/pp.120.3.739
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发表时间：
1999-07-01
期刊：
影响因子：
7.4
通讯作者：
Voelker, TA
中科院分区：
文献类型：
--
作者：
Knutzon, DS;Hayes, TR;Voelker, TA
加州月桂(Umbellularia Calfornica)12：0-酰基载体蛋白硫酯酶(BTE)在油菜(Brassica Napus)种子发育中的表达导致了月桂酸盐含量高达50%的油的产生。在这些BTE油中，月桂酸酯几乎只存在于三酰甘油的sn-1和sn-3位(T.A.Voelker，T.R.Hayes，A.C.Cranmer，H.M.Davies[1996]Factory J 9：229-241)。椰子(Cocos Nucifera)12：0-辅酶A-偏爱溶血磷脂酸酰基转移酶(D.S.Knutzon，K.D.Lardizabal，J.S.Nelsen，J.L.Bleibaum，H.M.Davies，J.G.Metz[1995]Plant Physiol 109：999-1006)在BTE油菜种子中的共表达促进了月桂酸酯在sn-2位的高效沉积，导致三黄酮苷的积累。将椰子蛋白导入月桂酸含量在50%以上的BTE油菜品系，进一步提高了总月桂酸水平。
Expression of a California bay laurel (Umbellularia californica) 12:0-acyl-carrier protein thioesterase, bay thioesterase (BTE), in developing seeds of oilseed rape (Brassica napus) led to the production of oils containing up to 50% laurate. In these BTE oils, laurate is found almost exclusively at the sn-1 and sn-3 positions of the triacylglycerols (T.A. Voelker, T.R. Hayes, A.C. Cranmer, H.M. Davies [1996] Plant J 9: 229-241). Coexpression of a coconut (Cocos nucifera) 12:0-coenzyme A-preferring lysophosphatitic acid acyltransferase (D.S. Knutzon, K.D. Lardizabal, J.S. Nelsen, J.L. Bleibaum, H.M. Davies, J.G. Metz [1995] Plant Physiol 109: 999-1006) in BTE oilseed rape seeds facilitates efficient laurate deposition at the sn-2 position, resulting in the acccumulation of trilaurin. The introduction of the coconut protein into BTE oilseed rape lines with laurate above 50 mol % further increases total laurate levels.