The Translation of Cyclin B1 and B2 is Differentially Regulated during Mouse Oocyte Reentry into the Meiotic Cell Cycle.
The Translation of Cyclin B1 and B2 is Differentially Regulated during Mouse Oocyte Reentry into the Meiotic Cell Cycle.
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DOI：
10.1038/s41598-017-13688-3
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发表时间：
2017-10-26
影响因子：
4.6
通讯作者：
Conti M
中科院分区：
文献类型：
--
作者：
Han SJ;Martins JPS;Yang Y;Kang MK;Daldello EM;Conti M
蛋白质周转的控制是减数分裂进程的关键。利用核糖核酸（RiboTag）免疫沉淀法、RNA结合蛋白免疫沉淀法和荧光素酶报告基因法，我们研究了小鼠卵母细胞中mRNA翻译、蛋白合成和降解率对细胞周期蛋白B1和B2稳态水平的影响。核糖体装载到Ccnb1和大多数mrna在细胞周期再进入期间增加，在生发囊泡破裂（GVBD）之后。随后是包含Mos或Ccnb1 3 '未翻译区域的报告基因的翻译，以及Mos和Cyclin B1蛋白的积累。相反，核糖体装载到Ccnb2 mRNA和Cyclin B2蛋白水平在减数分裂再入过程中发生最小的变化。细胞周期蛋白B1或B2在GV期的降解率是相当的。Mos和Ccnb1 mrna的翻译激活依赖于RNA结合蛋白CPEB1，而不是Ccnb2 mrna。抑制Cdk1活性，而不是Aurora A激酶活性，可以阻止Mos或Ccnb1报告基因的翻译，这表明它们在小鼠卵母细胞中的翻译需要MPF。相反，Ccnb2翻译对Cdk1抑制不敏感。因此，允许小鼠GV卵母细胞快速进入减数分裂的平衡状态可能是由两种细胞周期蛋白的差异翻译控制决定的。
Control of protein turnover is critical for meiotic progression. Using RiboTag immunoprecipitation, RNA binding protein immunoprecipitation, and luciferase reporter assay, we investigated how rates of mRNA translation, protein synthesis and degradation contribute to the steady state level of Cyclin B1 and B2 in mouse oocytes. Ribosome loading onto Ccnb1 and Mos mRNAs increases during cell cycle reentry, well after germinal vesicle breakdown (GVBD). This is followed by the translation of reporters containing 3′ untranslated region of Mos or Ccnb1 and the accumulation of Mos and Cyclin B1 proteins. Conversely, ribosome loading onto Ccnb2 mRNA and Cyclin B2 protein level undergo minimal changes during meiotic reentry. Degradation rates of Cyclin B1 or B2 protein at the GV stage are comparable. The translational activation of Mos and Ccnb1, but not Ccnb2, mRNAs is dependent on the RNA binding protein CPEB1. Inhibition of Cdk1 activity, but not Aurora A kinase activity, prevents the translation of Mos or Ccnb1 reporters, suggesting that MPF is required for their translation in mouse oocytes. Conversely, Ccnb2 translation is insensitive to Cdk1 inhibition. Thus, the poised state that allows rapid meiotic reentry in mouse GV oocytes may be determined by the differential translational control of two Cyclins.
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影响因子：
11.8
作者：
Gui, Liming;Homer, Hayden
通讯作者：
Homer, Hayden
影响因子：
4
作者：
Martins, Joao P. Sousa;Liu, Xueqing;Conti, Marco
通讯作者：
Conti, Marco
DOI：
10.1083/jcb.200607070
发表时间：
2007-01-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Marangos P;Verschuren EW;Chen R;Jackson PK;Carroll J
通讯作者：
Carroll J
影响因子：
4
作者：
Keady, Brian T.;Kuo, Peiwen;Hake, Laura E.
通讯作者：
Hake, Laura E.
影响因子：
16
作者：
Mendez, R;Murthy, KGK;Richter, JD
通讯作者：
Richter, JD