Human kidney free fatty acid and glucose uptake: evidence for a renal glucose fatty acid cycle
Human kidney free fatty acid and glucose uptake: evidence for a renal glucose fatty acid cycle
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DOI：
10.1152/ajpendo.1997.273.3.e650
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发表时间：
1997-09-01
影响因子：
5.1
通讯作者：
Gerich, J
中科院分区：
文献类型：
--
作者：
Meyer, C;Nadkarni, V;Gerich, J
为了确定游离脂肪酸（FFA）与人肾脏葡萄糖吸收之间的关系，12名吸收后正常志愿者接受了肾静脉导管插入术，并输注[6-H-3]葡萄糖和[9，10-H-3]棕榈酸酯至同位素稳态。动脉和肾静脉棕榈酸酯比活性无显著差异（3，533 +/-219 vs. 3，549 +/-220 dpm/μ mol，P = 0.64）。同位素测定的棕榈酸肾提取和摄取分数（7.2 +/-1.1%和9.1 +/-1.4 μ mol/min）与净平衡测量的结果（分别为8.3 +/-1.2%和9.7 +/-1.2 μ mol/min，P> 0.07和P> 0.7）无显著差异。肾棕榈酸摄取占其全身代谢的8.7 ± 1.3%。通过净平衡测量计算的肾亚油酸和油酸分数提取（分别为8.0 +/-0.9和7.7 +/-1.2%）彼此之间以及棕榈酸之间没有显著差异（均P> 0.7）。棕榈酸、亚油酸（7.9 +/-1.0 μ mol/min）和油酸（10.9 +/-2.0 μ mol/min）的肾摄取均与其动脉浓度成正比（r分别为0.70、0.68和0.63，均P <0.025）。肾葡萄糖摄取（93 +/-10 μ mol/min）占其全身代谢的12.6 +/-1.5%，与棕榈酸、亚油酸和油酸摄取的总和呈负相关（r =-0.76，P <0.01），这些数据表明，在吸收后人体中：1）肾脏是FFA和葡萄糖处置的重要部位，2）可能存在肾脏葡萄糖-脂肪酸循环，以及3）似乎很少或没有释放到储存的肾脏FFA的循环中。
To determine the relationship between free fatty acids (FFA) and glucose uptake by the human kidney, 12 postabsorptive normal volunteers underwent renal vein catheterization and were infused to isotopic steady state with [6-H-3]glucose and [9,10-H-3]palmitate. Arterial and renal vein palmitate specific activities were not significantly different (3,533 +/- 219 vs. 3,549 +/- 220 dpm/mu mol, P = 0.64). Palmitate renal fractional extraction and uptake determined isotopically (7.2 +/- 1.1% and 9.1 +/- 1.4 mu mol/min) were not significantly different fi om those calculated by net balance measurements (8.3 +/- 1.2% and 9.7 +/- 1.2 mu mol/min, P > 0.07 and P > 0.7, respectively). Renal palmitate uptake accounted for 8.7 +/- 1.3% of its systemic turnover. Renal linoleate and oleate fractional extraction calculated by net balance measurements (8.0 +/- 0.9 and 7.7 +/- 1.2%, respectively) were not significantly different from each other and that of palmitate (all P > 0.7). Renal uptake of palmitate, linoleate (7.9 +/- 1.0 mu mol/min), and oleate (10.9 +/- 2.0 mu mol/min) were all directly proportional to their arterial concentrations (r = 0.70, 0.68, and 0.63, respectively, all P < 0.025). Renal glucose uptake (93 +/- 10 mu mol/min) accounted for 12.6 +/- 1.5% of its systemic turnover and was inversely related to the sum of palmitate, linoleate, and oleate uptake (r = -0.76, P < 0.01), These data indicate that in postabsorptive humans: 1) the kidney is an important site of FFA and glucose disposal, 2) a renal glucose-fatty acid cycle may exist, and 3) there appears to be little or no release into the circulation of stored renal FFA.