Low-intensity Pulsed Ultrasound Improves Erectile Function in Streptozotocin-induced Type I Diabetic Rats
Low-intensity Pulsed Ultrasound Improves Erectile Function in Streptozotocin-induced Type I Diabetic Rats
复制标题
低强度脉冲超声改善链脲佐菌素诱导的 I 型糖尿病大鼠的勃起功能
DOI：
10.1016/j.urology.2015.07.026
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发表时间：
2015-12-01
期刊：
影响因子：
2.1
通讯作者：
Xin, Zhongcheng
中科院分区：
文献类型：
--
作者：
Lei, Hongen;Xin, Hua;Xin, Zhongcheng
目的 探讨低强度脉冲超声（LIPUS）治疗链脲佐菌素（STZ）诱导的 I 型糖尿病（DM）大鼠模型勃起功能障碍（ED）的效果。 材料与方法 70 只雄性 Sprague-Dawley 大鼠随机分为 2 个队列：正常对照组（NC）和 STZ 诱导的 DM 组，并进一步分为 2 组： DM、DM+LIPUS 100、DM+LIPUS 200 和 DM+LIPUS 300 组以及 DM+LESWT（低能冲击波疗法）300 阳性对照组。 LIPUS 亚组中的动物接受不同能量水平（100、200 和 300 mW/cm(2)）治疗 3 分钟，LESWT 组中的动物接受 300 次 0.09 mJ/mm(2) 电击。所有程序每周重复 3 次，持续 2 周。经过两周的清除期后，测量海绵体内压（ICP）；对阴茎中部区域进行组织学检查；通过蛋白质印迹分析检测阴茎组织中 VEGF、α SMA、eNOS 和 nNOS 的表达以及 TGF-β 1/Smad/CTGF 信号通路的活性。结果 LIPUS 治疗显着改善糖尿病大鼠的勃起功能，如 ICP 水平增强、内皮和平滑肌含量增加、I 型胶原/III 型胶原比例更高、弹性纤维数量增加以及 eNOS 和 nNOS 表达升高所证明。有趣的是，LIPUS 还与阴茎组织中 TGF-β 1/Smad/CTGF 信号通路的下调相关，该信号通路的激活与 ED 病理相关。 结论 LIPUS 治疗改善了 STZ 诱导的糖尿病大鼠的勃起功能并逆转了阴茎组织的病理变化。 LIPUS 疗法有潜力成为临床上糖尿病 ED 的无创疗法。 (C) 2015 爱思唯尔公司
OBJECTIVE To investigate the effect of low-intensity pulsed ultrasound (LIPUS) as a treatment for erectile dysfunction (ED) in a rat model of type I diabetes mellitus (DM) induced by streptozotocin (STZ).MATERIALS AND METHODS Seventy male Sprague-Dawley rats were randomly assigned to 2 cohorts: a normal control (NC) group and an STZ-induced DM group, which was further subdivided into DM, DM+LIPUS 100, DM+LIPUS 200, and DM+LIPUS 300 groups and a DM+LESWT (low-energy shock wave therapy) 300 positive control group. Animals in the LIPUS subgroups were treated at different energy levels (100, 200, and 300 mW/cm(2)) for 3 minutes, and animals in the LESWT group received 300 shocks at 0.09 mJ/mm(2). All procedures were repeated 3 times per week for 2 weeks. After a 2-week wash-out period, intracavernous pressure (ICP) was measured; the midpenile region was examined histologically; and VEGF, alpha SMA, eNOS, and nNOS expression, and activity of the TGF-beta 1/Smad/CTGF signaling pathway were examined in penile tissue by Western blot analysis.RESULTS LIPUS therapy significantly improved erectile function in diabetic rats, as evidenced by enhanced ICP levels, increased endothelial and smooth muscle content, a higher collagen I/collagen III ratio, increased quantity of elastic fibers, and elevated eNOS and nNOS expression. Interestingly, LIPUS was also associated with downregulation of the TGF-beta 1/Smad/CTGF signaling pathway in penile tissue, whose activation is correlated with ED pathology.CONCLUSION LIPUS therapy improved erectile function and reversed pathologic changes in penile tissue of STZ-induced diabetic rats. LIPUS therapy has potential as a noninvasive therapy for diabetic ED in the clinic. (C) 2015 Elsevier Inc.