Observation of dermal collagen fiber in wrinkled skin using polarization-resolved second-harmonic-generation microscopy
Observation of dermal collagen fiber in wrinkled skin using polarization-resolved second-harmonic-generation microscopy
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DOI：
10.1364/oe.17.000912
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发表时间：
2009-01-19
期刊：
影响因子：
3.8
通讯作者：
Araki, Tsutomu
中科院分区：
文献类型：
--
作者：
Yasui, Takeshi;Takahashi, Yu;Araki, Tsutomu
在抗衰老皮肤病学和皮肤化妆品开发等领域，用于皮肤光老化体内评估的光学探针方法是必要的。我们使用偏振分辨二次谐波发生 (SHG) 显微镜研究了紫外线 B 暴露 (UVB 暴露) 皮肤中皱纹方向和胶原蛋白取向之间的关系。 SHG 光的偏振各向异性图像表明，暴露于 UVB 的皮肤中的皱纹方向主要平行于真皮胶原纤维的方向。此外，在UVB暴露后几乎没有皱纹的皮肤中，胶原蛋白的取向从UVB暴露的皱纹皮肤的胶原蛋白取向改变为未暴露UVB的皮肤的胶原蛋白取向。所提出的方法有可能成为评估皮肤光老化的强大非侵入性工具。 (C) 2009 美国光学学会
Optical probe methods for in vivo assessments of cutaneous photoaging are necessary in fields such as anti-aging dermatology and skin cosmetic development. We investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved second-harmonic-generation (SHG) microscopy. A polarization anisotropic image of the SHG light indicated that wrinkle direction in UVB-exposed skin is predominantly parallel to the orientation of dermal collagen fibers. Furthermore, collagen orientation in post-UVB-exposed skin with few wrinkles changed from that of UVB-exposed wrinkled skin to that of no-UVB-exposed skin. The method proposed has the potential to become a powerful non-invasive tool for assessment of cutaneous photoaging. (C) 2009 Optical Society of America