Petroleum hydrocarbon-contaminated soil bioremediation assisted by isolated bacterial consortium and sophorolipid
Petroleum hydrocarbon-contaminated soil bioremediation assisted by isolated bacterial consortium and sophorolipid
复制标题
分离菌群和槐糖脂辅助石油烃污染土壤生物修复
DOI：
10.1016/j.envpol.2021.116476
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发表时间：
2021-01-20
影响因子：
8.9
通讯作者：
Fu, Rongbing
中科院分区：
文献类型：
--
作者：
Feng, Leiyu;Jiang, Xiupeng;Fu, Rongbing
石油烃对土壤的污染已成为一个全球性的环境问题。将分离的本地菌群与生物表面活性剂结合，提高了石油烃污染土壤的生物修复效果。土壤中总石油烃（TPH）的生物降解效率由12.2%提高到44.5%，土壤中总石油烃（TPH）的降解效率提高到57.7%，土壤中总石油烃（TPH）的降解效率提高到1.5 g sophorolidid /kg土壤。TPH降解过程的半衰期从分离联合体反应器的32.5 d缩短到分离联合体和1.5 g SL/kg干土的20.4 d。在污染土壤中添加生物表面活性剂促进了TPH从固体基质向水溶液的解吸和随后的增溶，最终提高了TPH在污染土壤中的生物利用度。生物表面活性剂还可以作为碳源，促进细胞生长和微生物活性，并通过共代谢加速生物降解过程。结果表明，在SL反应器中，酶活性和功能基因的数量都有所增加。生物表面活性剂提高了TPH的生物利用度，刺激了微生物活性，参与了共代谢。生物强化与SL相结合有利于石油烃污染土壤的生物修复。(C) 2021 Elsevier Ltd版权所有。
Pollution in soil by petroleum hydrocarbon has become a global environmental problem. The bioremediation of petroleum hydrocarbon-contaminated soil was enhanced with the combination of an isolated indigenous bacterial consortium and biosurfactant. The biodegradation efficiency of total petroleum hydrocarbon (TPH) was increased from 12.2% in the contaminated soil to 44.5% and 57.7% in isolated consortium and isolated consortium & 1.5 g sophorolipid (SL)/kg dry soil, respectively. The half-life of TPH degradation process was decreased from 32.5 d in the isolated consortium reactor to 20.4 d in the isolated consortium & 1.5 g SL/kg dry soil. The addition of biosurfactant into contaminated soils improved the TPH desorption from solid matrix to the aqueous solution and the subsequent solubilization, which ultimately improved the bioavailability of TPH in contaminated soils. Biosurfactant also served as carbon sources which contributed to the stimulation of cell growth and microbial activity and accelerated the biodegradation process via co-metabolism. The enzyme activities and quantities of functional genes were demonstrated to be incremented in SL reactors. The biosurfactant improved the TPH bioavailability, stimulated the microbial activities and participated in the co-metabolism. The combination of bioaugmentation and SL benefitted the bioremediation of petroleum hydrocarbon-contaminated soil. (C) 2021 Elsevier Ltd. All rights reserved.