Preclinical Characterization of 86/90Y-NM600 in a Variety of Murine and Human Cancer Tumor Models
Preclinical Characterization of 86/90Y-NM600 in a Variety of Murine and Human Cancer Tumor Models
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DOI：
10.2967/jnumed.118.224808
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发表时间：
2019-11-01
影响因子：
9.3
通讯作者：
Weichert, Jamey
中科院分区：
文献类型：
--
作者：
Grudzinski, Joseph J.;Hernandez, Reinier;Weichert, Jamey
We characterize the in vivo biodistribution and tumor selectivity of Y-86-NM600, a theranostic alkylphosphocholine radiometal chelate with broad tumor selectivity, in a variety of preclinical cancer models. Methods: Mice bearing flank tumors (representative of lung, pancreatic, prostate, liver, skin, and lymphoid cancers) were injected intravenously with 9.25 MBq of Y-86-NM600 and imaged longitudinally over 4-5 d using small-animal PET/CT. Percentage injected activity per gram (%IA/g) for each volume of interest was measured at each time point for the organs of interest. Mice were euthanized after the final time point, and the tumor and organs of interest were counted with an automatic.-counter. Absorbed doses delivered by Y-90-NM600 per injected activity (Gy/MBq) were estimated. Mice bearing B78 flank tumors were injected with a prescription of Y-90-NM600 that delivered 2.5 Gy of absorbed tumor dose and was compared with an equivalent absorbed dose delivered via external-beam radiotherapy using tumor volume as a measure of response. Histology and complete blood counts were analyzed in naive C57BL/6 mice that were injected with 9.25 MBq of Y-90-NM600 at 5, 10, and 28 d after injection. Results: PET imaging showed consistent tumor accumulation and retention across all tumor models investigated, with little off-target retention of NM600 except in the liver, as is characteristic of hepatobiliary metabolism. The tumor uptake was highest in the pancreatic and lymphoid cancer models, reaching peak concentrations of 9.34 +/- 2.66 %IA/g (n = 3) and 9.10 +/- 0.13 %IA/g (n = 3), respectively, at approximately 40-48 h after injection. These corresponded to tumor dose estimates of 2.72 +/- 0.33 Gy/MBq and 2.67 +/- 0.32 Gy/MBq, respectively. In the toxicity study, there were no visible signs of acute toxicity by histology, and perturbation of hematologic parameters was transient when observed, returning to pretherapy levels after 28 d. Conclusion: NM600 is a theranostic agent with a unique ability to selectively target a variety of cancer types, presenting a unique opportunity for PET image-guided targeted radionuclide therapy and combination with immunotherapies.
