ISOELECTRIC-FOCUSING WITH IMMOBILIZED PH GRADIENTS FOR THE ANALYSIS OF TRANSFERRIN (TF) SUBTYPES AND VARIANTS
ISOELECTRIC-FOCUSING WITH IMMOBILIZED PH GRADIENTS FOR THE ANALYSIS OF TRANSFERRIN (TF) SUBTYPES AND VARIANTS
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DOI：
10.1007/bf00279397
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发表时间：
1983-01-01
期刊：
影响因子：
5.3
通讯作者：
CLEVE, H
中科院分区：
文献类型：
--
作者：
GORG, A;WESER, J;CLEVE, H
通过等电聚焦 (IEF) 和固定 pH 梯度对人血清转铁蛋白 (Tf) 系统进行分析。为了证明遗传变异性，选择了 Fe1-Tf 区域。适合 Tf 系统分析的 pH 范围为 pH 5.20 至 pH 5.75。描述了常见 6 种 TfC 亚型的表型。未发现 TfC1、TfC2 和 TfC3 之间存在进一步的异质性。还提供了 TfC6 亚型以及 3 种不同的 TfB 和 3 种不同的 TfD 变体的表型。采用固定 pH 梯度的 IEF 似乎是分析遗传性 Tf 多态性的合适方法。
The human serum transferrin (Tf) system was analyzed by isoelectric focusing (IEF) with immobilized pH gradients. For the demonstration of the genetic variability the Fe1-Tf region was chosen. The pH range suitable for analysis of the Tf system was pH 5.20 to pH 5.75. The phenotypes of the common 6 TfC subtypes are described. No further heterogeneity among TfC1, TfC2 and TfC3 was noted. Also presented are the phenotypes of the TfC6 subtype, and of 3 different TfB and 3 different TfD variants. IEF with immobilized pH gradients appears to be a suitable method for the analysis of the inherited Tf polymorphism.