Temporal changes in the hypothalamic and serum luteinizing hormone-releasing hormone (LH-RH) levels and the circulating ovarian steroids during the rat oestrous cycle.
Temporal changes in the hypothalamic and serum luteinizing hormone-releasing hormone (LH-RH) levels and the circulating ovarian steroids during the rat oestrous cycle.
复制标题
大鼠发情周期期间下丘脑和血清黄体生成素释放激素（LH-RH）水平以及循环卵巢类固醇的时间变化。
DOI：
10.1530/acta.0.0850449
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
S. Kalra
中科院分区：
文献类型：
--
作者：
P. Kalra;S. Kalra
在4天发情周期的不同时间处死周期雌性大鼠。用放射免疫分析法测定下丘脑内侧基底部（MBH）的LH-RH和血清LH-RH、LH、雌二醇（Oe 2）和孕酮。MBH LH-RH含量在发情前期19.55-20.15 h最低，在发情期和发情间期I逐渐升高，发情间期II中午显著升高，18.00 h急剧下降。虽然从发情前期午夜到发情间期II血清LH水平保持基础水平，但发情期12.00和21.00 h血清LH-RH水平显著升高（与发情前期午夜水平相比），发情间期I 15.00和22.00 h之间下降。结合高Oe 2滴度，在MBH和血清LH-RH最初下降，在发情前期14.07-14.45小时之间，然后突然增加之间的14.55-15.55小时;排卵前血清LH的上升，观察从16.05小时起。MBH和血清LH-RH活性在19.55-24.00 h逐渐下降至午后水平，血清LH活性在17.05-18.05 h达到高峰。这些研究表明，MBH LH-RH的高分泌（合成+释放）可能是LH排卵前释放的原因。
Cycling female rats were sacrificed at various times during the 4-day oestrous cycle. LH-RH in the medial basal hypothalamus (MBH) and serum LH-RH, LH, oestradiol (Oe2), and progesterone were analyzed by radioimmunoassays. The MBH LH-RH content was lowest at 19.55-20.15 h during pro-oestrus but increased gradually through oestrus and dioestrus I to significantly higher values at noon of dioestrus II, and then decreased precipitously at 18.00 h. Although serum LH levels remained basal from midnight of pro-oestrus through dioestrus II, serum LH-RH levels were significantly elevated at 12.00 and 21.00 h on oestrus (vs. the midnight pro-oestrus levels) and declined between 15.00 and 22.00 h of dioestrus I. In conjunction with high Oe2 titres, LH-RH in the MBH and serum declined initially during pro-oestrus between 14.07-14.45 h and then increased abruptly between 14.55-15.55 h; the pre-ovulatory rise in serum LH was observed from 16.05 h onwards. LH-RH activity in the MBH and serum receded gradually to the early afternoon levels by 19.55-24.00 h while the peak in serum LH was observed at 17.05-18.05 h. These studies suggested that the hypersecretion of the MBH LH-RH (synthesis + release) may be responsible for the pre-ovulatory discharge of LH.