Direct electrochemistry of cytochrome c immobilized on one dimensional Au nanoparticles functionalized magnetic N-doped carbon nanotubes and its application for the detection of H2O2
Direct electrochemistry of cytochrome c immobilized on one dimensional Au nanoparticles functionalized magnetic N-doped carbon nanotubes and its application for the detection of H2O2
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DOI：
10.1016/j.snb.2018.11.005
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
Min Zhang;Jing Zheng;Jianping Wang;Jingli Xu;T. Hayat;N. S. Alharbi
中科院分区：
文献类型：
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作者：
Min Zhang;Jing Zheng;Jianping Wang;Jingli Xu;T. Hayat;N. S. Alharbi
近年来，用于各种新型生物传感器的贵金属功能化磁性碳纳米管的制备由于其协同提高的灵敏度而引起了人们的极大兴趣。该论文将Fe3O4和Cu纳米粒子（NPs）集成到NCNT中，通过一锅高温分解过程获得氮掺杂碳@Fe3O4-Cu纳米管（NCNTs@Fe3O4@Cu）。然后，将Au NPs组装到磁性NCNT上，通过Cu NPs的电镀置换获得NCNTs@Fe3O4@Au复合材料。所得复合材料为酶固定化提供了一个友好的平台，以开发高灵敏度的生物传感器。当NCNTs@Fe3O4@Au复合材料积累细胞色素c(cyt c)后，在外部磁铁的作用下，cyt c/NCNTs@Fe3O4@Au聚集到电极表面。因此，设计的生物传感器的灵敏度可以进一步提高，并且可以排除大部分电化学干扰。得益于复合材料独特的杂化结构，细胞色素c的直接电子转移得到了高度促进，并且构建的生物传感器在检测H2O2方面表现出良好的性能，检测限极低，为0.3μM，这揭示了制造新型电化学生物传感器的潜在应用。
In recent years, the fabrication of noble metal functionalized magnetic carbon nanotubes for a variety of novel biosensors has aroused considerable interest owing to their synergistic improved sensitivity. In the paper, the Fe3O4and Cu nanoparticles (NPs) were integrated into the NCNTs to obtain N-doped carbon@Fe3O4-Cu nanotubes (NCNTs@Fe3O4@Cu) through a one-pot high temperature decomposition procedure. Then, Au NPs were assembled on the magnetic NCNTs to obtain NCNTs@Fe3O4@Au composite by galvanic replacement with Cu NPs. The resultant composite had provided a friendly platform for enzyme immobilization to develop highly sensitive biosensors. After the cytochrome c(cyt c) was accumulated by NCNTs@Fe3O4@Au composite, the cyt c/NCNTs@Fe3O4@Au gathered to the surface of electrode with an external magnet. Thus, the sensitivity of designed biosensor could be further improved, and most of electrochemical interferences could be excluded. Benefiting from the unique hybrid structure of the composite, the direct electron transfer of cyt c was highly facilitated and the constructed biosensors exhibited good performance for the detection of H2O2with an extraordinary low detection limit of 0.3 μM, which revealed potential application for fabricating novel electrochemical biosensors.