Flow cytometric deoxyribonucleic acid analysis of granulosa cells aspirated from human ovarian follicles. A new method to distinguish healthy and atretic ovarian follicles.
Flow cytometric deoxyribonucleic acid analysis of granulosa cells aspirated from human ovarian follicles. A new method to distinguish healthy and atretic ovarian follicles.
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对从人卵巢卵泡中吸出的颗粒细胞进行流式细胞术脱氧核糖核酸分析。
DOI：
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发表时间：
1982
影响因子：
5.8
通讯作者：
Anne Grete Byskov
中科院分区：
文献类型：
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作者：
L. Westergaard;P. Mcnatty;I. J. Christensen;Jørgen K. Larsen;Anne Grete Byskov
通过流式细胞术分析人卵泡的颗粒细胞抽吸物，以确定有丝分裂细胞周期的DNA S期细胞的分数。该研究的目的是评估S期颗粒细胞的百分比（S分数）是否可用于指示卵泡是否健康或闭锁。卵泡液中S组分与雌二醇浓度呈显著正相关（r = 0.6，P <0.001）。超过85%的卵泡具有16%或更高的S-分数包含卵泡内雌二醇水平等于或大于200 ng/ml，并具有低雄烯二酮：雌二醇比。相反，95%或更多的卵泡的S-分数小于16%，含有低雌二醇（小于200 ng/ml），并有一个高雄烯二酮雌二醇比。我们的结论是，流式细胞术DNA测量卵泡吸出物提供了一个可靠和快速的方法来区分健康和闭锁的卵泡。由于S期分析只需要整个颗粒细胞群的一小部分（小于5%），因此该技术可以立即获得大部分细胞或进行其他生化研究。此外，由于避免了切除卵巢组织，该技术可能适用于卵巢功能正常但因某种原因接受剖腹手术或腹腔镜检查的女性的研究。
Granulosa cell aspirates from human ovarian follicles were analyzed by flow cytometry to determine the fraction of cells in the DNA S-phase of the mitotic cell cycle. The aim of the study was to evaluate if the percentage of granulosa cells in S-phase (the S-fraction) could be used to indicate whether a follicle was healthy or atretic. A highly significant relationship was found between the S-fraction and the concentration of estradiol in the follicular fluid (r = 0.6, P less than 0.001). More than 85% of the follicles having an S-fraction of 16% or greater contained intrafollicular levels of estradiol equal to or greater than 200 ng/ml and had a low androstenedione:estradiol ratio. Conversely, 95% or more of the follicles that had an S-fraction of less than 16% contained low estradiol (less than 200 ng/ml) and had a high androstenedione to estradiol ratio. We conclude that flow cytometric DNA measurements on follicular aspirates provide a reliable and rapid method by which to distinguish healthy and atretic ovarian follicles. Since only a small fraction (less than 5%) of an entire granulosa cell population is required for S-phase analysis, the technique allows the majority of cells to be immediately available or other biochemical studies. Moreover, since excision of ovarian tissue is avoided, the technique may be acceptable for studies on women with normal ovarian function but who are undergoing laparotomy or laparoscopy for some reason.