Quantification and molecular characterization of regulatory T cells in connective tissue diseases
Quantification and molecular characterization of regulatory T cells in connective tissue diseases
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DOI：
10.1080/08916930802282651
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发表时间：
2009-01-01
期刊：
影响因子：
3.5
通讯作者：
Matache, Cristiana
中科院分区：
文献类型：
--
作者：
Banica, Leontina;Besliu, Alina;Matache, Cristiana
我们研究的目的是调查和表征结缔组织疾病（系统性红斑狼疮、系统性硬化症、干燥综合征、多发性皮肌炎）患者外周血中的调节性 T 细胞 (Treg)，并与健康对照的血液进行比较。通过流式细胞术对 Treg 细胞进行定量和表型表征，同时通过实时 PCR 评估 Foxp3 mRNA 的表达水平。无论结缔组织疾病的类型如何，患者外周血 Treg 细胞的百分比均低于对照组。 Treg 细胞，尤其是那些表达一种表型标记物的 Treg 细胞，似乎不仅在患者和健康对照之间存在差异，而且在疾病类型之间也存在差异。此外，自身抗体的存在以及疾病活动似乎与特定的 Treg 细胞群相关，尤其是那些表达所检查的表型标记物之一的 Treg 细胞群。与治疗的相关性表明，糖皮质激素加抗疟药或其他免疫抑制药物会降低 Treg 细胞的百分比，尤其是具有记忆表型的 Treg 细胞的百分比。这些发现表明 Treg 细胞水平存在失调，并表明这些细胞参与结缔组织疾病的病理学。此外，我们的数据与 Treg 细胞可能成为某些自身免疫性疾病的治疗靶点的建议一致。
The aim of our study was to investigate and characterize regulatory T cells (Treg) in peripheral blood of patients with connective tissue diseases (Systemic lupus erythematosus, systemic sclerosis, Sjogren's syndrome, poly- and dermatomyositis) as compared with blood from healthy controls. Treg cells were quantified and phenotypically characterized by flow cytometry while the expression level of Foxp3 mRNA was evaluated by real time PCR. A reduced percentage of peripheral blood Treg cells was found in patients than in controls, irrespective of the type of connective tissue disease. Treg cells, especially those expressing one of the phenotypical markers, seemed to differ not only between patients and healthy controls but also among types of diseases. Additionally, the presence of autoantibodies as well as disease activity appeared to be correlated with particular Treg cell populations, especially those expressing one of the examined phenotypical markers. Correlations with therapy suggested that glucocorticoids plus antimalarial or other immunosuppressor drugs diminished the percentage of Treg cells, especially of those with memory phenotype. These findings indicated dysregulations at the level of Treg cells and suggested an involvement of these cells in the pathology of connective tissue diseases. Moreover, our data are in agreement with the suggestion that Treg cells could be therapeutic targets for some autoimmune diseases.