Effects of glutamine on hydrogen peroxide-induced oxidative damage in intestinal epithelial cells of Jian carp (Cyprinus carpio var.Jian)
Effects of glutamine on hydrogen peroxide-induced oxidative damage in intestinal epithelial cells of Jian carp (Cyprinus carpio var.Jian)
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谷氨酰胺对过氧化氢诱导的建鲤肠上皮细胞氧化损伤的影响
DOI：
10.1016/j.aquaculture.2008.10.053
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发表时间：
2009-03-20
期刊：
影响因子：
4.5
通讯作者：
Jiang, Jun
中科院分区：
文献类型：
--
作者：
Chen, Jin;Zhou, Xiao-qiu;Jiang, Jun
本研究探讨了谷氨酰胺对过氧化氢（H2O2）诱导的鲤鱼离体肠细胞氧化应激的影响。为了选择诱导肠细胞氧化应激的最佳H2O2浓度，采用不同浓度H2O2 （0-100 μ M）处理培养16 h。结果表明，H2O2暴露使培养基中乳酸脱氢酶活性和丙二醛水平呈剂量依赖性增加，表明H2O2毒性增加。因此，100 μ M为诱导氧化应激的适宜浓度。然后我们检查了氧化应激条件下谷氨酰胺的细胞保护作用。细胞在H2O2 （100 μ M）存在下用谷氨酰胺（0-20 mM）处理16 h，对照细胞仅在不含谷氨酰胺的MEM中保存。结果表明，谷氨酰胺完全阻断h2o2刺激下乳酸脱氢酶的释放。此外，谷氨酰胺将h2o2诱导的丙二醛水平和蛋白质羰基含量的增加降低到对照培养的水平。此外，谷氨酰胺处理完全阻止了H2O2诱导的Na+-K+- atp酶、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽s -转移酶活性的降低以及还原性谷胱甘肽浓度和还原性谷胱甘肽与氧化性谷胱甘肽比值的降低。在H2O2存在下用谷氨酰胺培养的细胞与对照细胞在上述大多数标记物的数值上没有显著差异。谷氨酰胺浓度仅为4 mM时，对鲤鱼肠上皮细胞具有完全的保护作用，表明谷氨酰胺对h2o2诱导的氧化应激具有保护作用。爱思唯尔B.V.版权所有
The present study explored the effects of glutamine on hydrogen peroxide (H2O2)-induced oxidative stress in isolated carp enterocytes. In order to select an optimal H2O2 concentration to induce oxidative stress in enterocytes, cultures were treated with different concentrations of H2O2 (0-100 mu M) for 16 h. The results showed that exposure to H2O2 increased lactate dehydrogenase activity and malondialdehyde levels in a dose-dependent manner in the culture medium, suggesting increase toxicity of H2O2. Thus, 100 mu M was an appropriate concentration for inducing oxidative stress. We then examined the cytoprotective effects of glutamine under conditions of oxidative stress. Cells were treated with glutamine (0-20 mM) in the presence of H2O2 (100 mu M) for 16 h. The control cells were kept in the glutamine-free MEM only. Results showed that glutamine completely blocked H2O2-stimulated release of lactate dehydrogenase. Furthermore, glutamine reduced H2O2-induced increase in malondialdehyde level and protein carbonyls content to the level seen in the control culture. In addition, glutamine treatment completely prevented the decrease in Na+-K+-ATPase, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase activities as well as reduced glutathione concentration and the ratio between reduced and oxidized glutathione induced by H2O2. No significant difference was observed between cells incubated with glutamine in the presence of H2O2 and control cells in values for most of the markers mentioned above. Complete protection was obtained at a concentration of glutamine as small as 4 mM. The present results indicate that glutamine is effective in protecting against H2O2-induced oxidative stress in carp intestinal epithelial cells. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.