ITIH3 is a potential biomarker for early detection of gastric cancer.
ITIH3 is a potential biomarker for early detection of gastric cancer.
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DOI：
10.1021/pr100192h
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发表时间：
2010-07-02
影响因子：
4.4
通讯作者：
Lim, Yoon Pin
中科院分区：
文献类型：
--
作者：
Chong, Poh Kuan;Lee, Huiyin;Zhou, Jianbiao;Liu, Shaw-Cheng;Loh, Marie Chiew Shia;Wang, Ting Ting;Chan, Siew Pang;Smoot, Duane T.;Ashktorab, Hassan;So, Jimmy Bok Yan;Lim, Khong Hee;Yeoh, Khay Guan;Lim, Yoon Pin
胃癌是世界上发病率和死亡率最高的疾病之一。早期发现是改善胃癌患者预后的关键措施。为了鉴定胃癌检测的潜在标志物，我们将异种移植小鼠模型与血浆蛋白质组学方法相结合。裸鼠皮下注射MKN45胃癌细胞，收集不同肿瘤大小小鼠的血浆样本，进行iTRAQ和质谱分析。与对照组相比，ITIH3蛋白在荷瘤小鼠血浆中的表达更高。随后对167例临床血浆样本（83例无癌患者和84例胃癌患者）进行了ITIH3表达筛查，发现胃癌患者血浆中ITIH3表达水平较高。根据受试者工作特征（ROC）曲线估计，ih3在胃癌检测中的最大灵敏度为96%，特异性为66%。此外，早期胃癌患者血浆中iht3水平明显高于非胃癌患者（p < 0.001）。我们的数据表明，ih3可能是一个有用的早期检测胃癌的生物标志物。
Gastric cancer has one of the highest morbidities and mortalities worldwide. Early detection is key measure to improve the outcome of gastric cancer patients. In our efforts to identify potential markers for gastric cancer detection, we coupled xenotransplantation mouse model with a plasma proteomic approach. MKN45 gastric cancer cells were subcutaneously injected into nude mice and plasma samples from mice bearing different sizes of tumors were collected and subjected to iTRAQ and mass spectrometry analysis. ITIH3 protein was found to be more highly expressed in plasma of tumor bearing mice compared to control. Subsequent screening of ITIH3 expression in 167 clinical plasma samples, including 83 cancer-free subjects and 84 gastric cancer patients, revealed higher ITIH3 level in the plasma of gastric cancer patients. A receiver operating characteristics (ROC) curve estimated a maximal sensitivity of 96% at 66% specificity for ITIH3 in gastric cancer detection. In addition, plasma from early stage gastric cancer patient has significantly (p < 0.001) higher level of ITIH3 compared to that from noncancer subject. Our data suggest that ITIH3 may be a useful biomarker for early detection of gastric cancer.
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影响因子：
15.8
作者：
Faca VM;Song KS;Wang H;Zhang Q;Krasnoselsky AL;Newcomb LF;Plentz RR;Gurumurthy S;Redston MS;Pitteri SJ;Pereira-Faca SR;Ireton RC;Katayama H;Glukhova V;Phanstiel D;Brenner DE;Anderson MA;Misek D;Scholler N;Urban ND;Barnett MJ;Edelstein C;Goodman GE;Thornquist MD;McIntosh MW;DePinho RA;Bardeesy N;Hanash SM
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Hanash SM
影响因子：
4.8
作者：
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通讯作者：
TERAO, T
影响因子：
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作者：
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影响因子：
4.4
作者：
Gan, Chee Sian;Chong, Poh Kuan;Wright, Phillip C.
通讯作者：
Wright, Phillip C.
影响因子：
4.4
作者：
Ho, Jiapei;Kong, Jacklyn-Wai-Fun;Lim, Yoon-Pin
通讯作者：
Lim, Yoon-Pin