Expression profiling of glycosyltransferases and related enzymes using gene microarrays
Expression profiling of glycosyltransferases and related enzymes using gene microarrays
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DOI：
10.1016/s0076-6879(06)16010-3
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发表时间：
2006-01-01
期刊：
影响因子：
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通讯作者：
Head, Steven R.
中科院分区：
文献类型：
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作者：
Hammond, Jennifer A.;Head, Steven R.
基因表达分析已成为研究疾病过程、生长和发育的生物学模型的标准工具，并在临床研究领域得到越来越多的应用。人类基因组序列的完成为糖组学研究界提供了与相关领域（如质谱聚糖分析和蛋白质组学）发展协同作用的工具。利用微阵列技术测量基因表达的变化是研究糖基转移酶、碳水化合物结合蛋白和相关酶表达谱的一种有用方法。标准化微阵列平台的可用性，用于样品制备和杂交的高质量试剂，以及图像和统计分析工具，促进了该技术在糖生物学应用中的最新进展。今天，大多数微阵列实验都是在专门的核心设施中完成的，这些设施配备了仪器、软件和具有应用专业知识的人员。本章的重点是所有平台和糖生物学家领域内常见的微阵列分析方面：考虑实验设计问题和从各种适合微阵列分析的组织来源制备RNA样品的方法。
Gene expression analysis has become a standard tool for studies of biological models of disease processes, growth, and development and has been used increasingly in the field of clinical research. The completion of the human genome sequence is now providing the glycomics research community with the tools to develop a synergism with related fields such as MS glycan profiling and proteomics. The use of microarray technology to measure changes in gene expression is a useful approach for the study of the expression profiles of glycosyltransferases, carbohydrate binding proteins, and related enzymes. The availability of standardized microarray platforms, high-quality reagents for sample preparation and hybridization, and image and statistical analysis tools has contributed to recent advances in the application of this technology to glycobiology. Most microarray experiments today are done within specialized core facilities equipped with instrumentation, software, and personnel with expertise in their application. The focus of this chapter is on aspects of microarray analysis common to all platforms and within the realm of the glycobiologist: consideration of experimental design issues and methods for the preparation of RNA samples from a variety of tissue sources suitable for microarray analysis.