CELL-DEATH IN COLORECTAL POLYPS AS EVALUATED BY IN-SITU 3'-TAILING REACTION AND ITS RELATIONSHIP TO BCL-2 EXPRESSION
CELL-DEATH IN COLORECTAL POLYPS AS EVALUATED BY IN-SITU 3'-TAILING REACTION AND ITS RELATIONSHIP TO BCL-2 EXPRESSION
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DOI：
10.1111/j.1440-1827.1995.tb03388.x
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发表时间：
1995-10-01
影响因子：
2.2
通讯作者：
NARIYA, S
中科院分区：
文献类型：
--
作者：
NAKAMURA, T;SAKAI, T;NARIYA, S
从细胞死亡的角度，通过使用原位3 '-加尾反应（ISTR）（其鉴定细胞死亡相关的DNA双链断裂）和bcl-2癌蛋白（BCL-2）和Ki-67的免疫组织化学分析结直肠息肉。在非肿瘤性腺体中存在很少的ISTR阳性细胞，而38%的紧邻腺瘤的非肿瘤性粘膜具有许多标记的核，提示与肿瘤替代相关的细胞死亡。肿瘤腺体含有不等数量的ISTR阳性细胞核，大多数显示凋亡小体的形态特征。在具有最显著ISTR标记的代表性腺体中，其指数与腺体的分级、增殖活性无显著相关性。（Ki-67标记指数）或BCL-2阳性。但在每种情况下，无或很少有ISTR标记核的肿瘤腺体倾向于强烈表达BCL-2，所有瘤或癌组织中，免疫组织化学染色阳性率大于15%的，均呈弱阳性反应。总体而言，BCL-2在腺瘤中的表达明显强于非肿瘤性粘膜和癌，尽管腺瘤和癌之间的ISTR标记没有显著差异。这些结果表明，大肠肿瘤性息肉中的细胞死亡对其生长速率没有显著影响，BCL-2至少部分地在细胞凋亡的调节中起一定作用。
Colorectal polyps were analyzed from the standpoint of cell death by using the in situ 3'-tailing reaction (ISTR), which identifies cell death-associated DNA double strand breaks, and immunohistochemistry for bcl-2 oncoprotein (BCL-2) and for Ki-67, There were few ISTR-positive cells in the nonneoplastic glands, whereas 38% of non-neoplastic mucosa just adjacent to the adenoma had many labeled nuclei, suggestive of cell death associated with replacement by tumor. The neoplastic glands contained variable number of ISTR-positive nuclei, mostly showing the morphological feature of apoptotic bodies. In the representative glands with the most prominent ISTR-labeling, their indices did not have a significant relationship to the grade of atypia, to the proliferative activity (Ki-67 labeling indices) of neoplastic glands, or to BCL-2 stainability, In each case, however, the neoplastic glands with no or few ISTR-labeled nuclei tended to express BCL-2 intensely, and all lesions of adenoma or carcinoma with more than 15% of ISTR-labeling indices showed weak BCL-2 immunoreactivity. In general, BCL-2 expression was significantly stronger in the adenoma than in non-neoplastic mucosa and carcinoma, although there was no significant difference of ISTR-labeling between adenoma and carcinoma, These results indicate that cell death in colorectal neoplastic polyps does not have a significant influence on their growth rate, and that BCL-2 plays some role, at least in part, in the regulation of apoptosis.