Glucosinolate engineering identifies γ-glutamyl peptidase
Glucosinolate engineering identifies γ-glutamyl peptidase
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DOI：
10.1038/nchembio.185
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发表时间：
2009-08-01
影响因子：
14.8
通讯作者：
Halkier, Barbara Ann
中科院分区：
文献类型：
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作者：
Geu-Flores, Fernando;Nielsen, Morten Thrane;Halkier, Barbara Ann
食用十字花科蔬菜与降低患癌症的风险有关，这一现象归因于这些蔬菜所特有的硫代葡萄糖苷。我们报道了通过代谢工程在非十字花科植物benthamiana中生产生物活性的苄基硫代葡萄糖苷。该研究包括鉴定γ -谷氨酰基肽酶1 (GGP1)，该酶通过代谢积累的谷胱甘肽偶联物大大增加了硫代葡萄糖苷的产量，这是一种以前未被描述的硫代葡萄糖苷生物合成或含有谷氨酰胺氨基转移酶结构域的蛋白质的活性。
Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.